TrxB

• Description: thioredoxin reductase (NADPH)
  
  

Gene name	trxB
Synonyms	yvcH
Essential	yes PubMed
Product	thioredoxin reductase (NADPH)
Function	keeps thioredoxin in the reduced state
Gene expression levels in SubtiExpress: trxB
MW, pI	34 kDa, 4.99
Gene length, protein length	948 bp, 316 aa
Immediate neighbours	yvcI, cwlO
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

electron transport/ other, resistance against oxidative and electrophile stress, essential genes

This gene is a member of the following regulons

Spx regulon

The gene

Basic information

• Locus tag: BSU34790

Phenotypes of a mutant

essential PubMed

Database entries

• BsubCyc: BSU34790

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Thioredoxin + NADP⁺ = thioredoxin disulfide + NADPH (according to Swiss-Prot)

• Protein family: class-II pyridine nucleotide-disulfide oxidoreductase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:

• Localization:
  • cytoplasm (according to Swiss-Prot)

Database entries

• BsubCyc: BSU34790

• Structure:

• UniProt: P80880

• KEGG entry: [2]

• E.C. number: 1.8.1.9

Expression and regulation

• Operon:

• Expression browser: trxB PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:
  • Spx: transcription activation PubMed

• Additional information:
  • A ncRNA is predicted between yvcI and trxB PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 434 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 1246 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Jun Lu, Arne Holmgren
The thioredoxin antioxidant system.
Free Radic Biol Med: 2014, 66;75-87
[PubMed:23899494] [WorldCat.org] [DOI] (I p)