ArgS

From SubtiWiki
Revision as of 09:41, 17 April 2014 by 134.76.70.252 (talk)
Jump to: navigation, search
Gene name argS
Synonyms
Essential yes PubMed
Product arginyl-tRNA synthetase
Function translation
Gene expression levels in SubtiExpress: argS
Metabolic function and regulation of this protein in SubtiPathways:
argS
MW, pI 62 kDa, 5.11
Gene length, protein length 1668 bp, 556 aa
Immediate neighbours narK, ywiB
Sequences Protein DNA DNA_with_flanks
Genetic context
ArgS context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
ArgS expression.png




























Categories containing this gene/protein

translation, essential genes, universally conserved proteins

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU37330

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + L-arginine + tRNA(Arg) = AMP + diphosphate + L-arginyl-tRNA(Arg) (according to Swiss-Prot)
  • Protein family: class-I aminoacyl-tRNA synthetase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 3FNR (from Campylobacter jejuni, 39% identity, 56% similarity)
  • KEGG entry: [3]

Additional information

universally conserved protein

Expression and regulation

  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 1206 PubMed
    • number of protein molecules per cell (complex medium with amino acids, without glucose): 3289 PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

E Presecan, I Moszer, L Boursier, H Cruz Ramos, V de la Fuente, M-F Hullo, C Lelong, S Schleich, A Sekowska, B H Song, G Villani, F Kunst, A Danchin, P Glaser
The Bacillus subtilis genome from gerBC (311 degrees) to licR (334 degrees).
Microbiology (Reading): 1997, 143 ( Pt 10);3313-3328
[PubMed:9353933] [WorldCat.org] [DOI] (P p)