HisS

• Description: histidyl-tRNA synthetase
  
  

Gene name	hisS
Synonyms
Essential	yes PubMed
Product	histidyl-tRNA synthetase
Function	translation
Gene expression levels in SubtiExpress: hisS
Metabolic function and regulation of this protein in SubtiPathways: hisS
MW, pI	47 kDa, 5.229
Gene length, protein length	1272 bp, 424 aa
Immediate neighbours	aspS, yrzK
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

translation, essential genes

This gene is a member of the following regulons

T-box

The gene

Basic information

• Locus tag: BSU27560

Phenotypes of a mutant

essential PubMed

Database entries

• BsubCyc: BSU27560

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + L-histidine + tRNA(His) = AMP + diphosphate + L-histidyl-tRNA(His) (according to Swiss-Prot)

• Protein family: class-II aminoacyl-tRNA synthetase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • cytoplasm (according to Swiss-Prot)

Database entries

• BsubCyc: BSU27560

• Structure: 1H4V (from Thermus thermophilus, 43% identity, 61% similarity) PubMed

• UniProt: O32039

• KEGG entry: [2]

• E.C. number: 6.1.1.21

Expression and regulation

• Operon: hisS-aspS

• Expression browser: hisS PubMed

• Sigma factor:

• Regulation:
  • induced by histidine or aspartate limitation (T-box) PubMed

• Regulatory mechanism:
  • T-box: RNA switch, transcriptional antitermination PubMed

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 990 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 2240 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Ana Gutiérrez-Preciado, Tina M Henkin, Frank J Grundy, Charles Yanofsky, Enrique Merino
Biochemical features and functional implications of the RNA-based T-box regulatory mechanism.
Microbiol Mol Biol Rev: 2009, 73(1);36-61
[PubMed:19258532] [WorldCat.org] [DOI] (I p)