AspB

From SubtiWiki
Revision as of 09:28, 17 April 2014 by 134.76.70.252 (talk)
Jump to: navigation, search
  • Description: aspartate transaminase

Gene name aspB
Synonyms
Essential no
Product aspartate transaminase
Function biosynthesis of aspartate
Gene expression levels in SubtiExpress: aspB
Metabolic function and regulation of this protein in SubtiPathways:
aspB
MW, pI 42 kDa, 5.298
Gene length, protein length 1179 bp, 393 aa
Immediate neighbours asnS, ypmB
Sequences Protein DNA DNA_with_flanks
Genetic context
AspB context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
AspB expression.png















Categories containing this gene/protein

biosynthesis/ acquisition of amino acids, most abundant proteins

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU22370

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate (according to Swiss-Prot)
  • Protein family: TCR/tet family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Modification:
  • Effectors of protein activity:

Database entries

  • Structure: 1J32 (from Phormidium lapideum, 45% identity, 66% similarity)
  • KEGG entry: [2]

Additional information

Expression and regulation

  • Regulation: constitutive
  • Regulatory mechanism:
  • Additional information:
    • belongs to the 100 most abundant proteins PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 10916 PubMed
    • number of protein molecules per cell (complex medium with amino acids, without glucose): 13715 PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Christine Eymann, Annette Dreisbach, Dirk Albrecht, Jörg Bernhardt, Dörte Becher, Sandy Gentner, Le Thi Tam, Knut Büttner, Gerrit Buurman, Christian Scharf, Simone Venz, Uwe Völker, Michael Hecker
A comprehensive proteome map of growing Bacillus subtilis cells.
Proteomics: 2004, 4(10);2849-76
[PubMed:15378759] [WorldCat.org] [DOI] (P p)