NrnA

Description: oligoribonuclease (nanoRNase), 3',5'-bisphosphate nucleotidase

Gene name	nrnA
Synonyms	ytqI
Essential	no
Product	oligoribonuclease (nanoRNase), 3',5'-bisphosphate nucleotidase
Function	degradation of RNA oligonucleotides,
MW, pI	34 kDa, 4.668
Gene length, protein length	939 bp, 313 aa
Immediate neighbours	ytzJ, ytpI
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

Rnases

The gene

Basic information

• Locus tag: BSU29250

Phenotypes of a mutant

The mutant requires cysteine PubMed

Database entries

• BsubCyc: BSU29250

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • Adenosine 3',5'-bisphosphate + H₂O = adenosine 5'-phosphate + phosphate (according to Swiss-Prot)
  • oligoribonuclease (nanoRNAse), 3',5'-bisphosphate nucleotidase PubMed
  • hydrolyze oligo(deoxy)ribonucleotides in a 5'-3' direction PubMed

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • Cytoplasm (Homogeneous) PubMed

Database entries

• BsubCyc: BSU29250

• Structure:

• UniProt: O34600

• KEGG entry: [3]

• E.C. number: 3.1.3.7

Expression and regulation

• Operon: nrnA PubMed

• Expression browser: nrnA PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 950 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 1111 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Claudiu M Nelersa, Brad J Schmier, Arun Malhotra
Purification and crystallization of Bacillus subtilis NrnA, a novel enzyme involved in nanoRNA degradation.
Acta Crystallogr Sect F Struct Biol Cryst Commun: 2011, 67(Pt 10);1235-8
[PubMed:22102036] [WorldCat.org] [DOI] (I p)

Taisuke Wakamatsu, Kwang Kim, Yuri Uemura, Noriko Nakagawa, Seiki Kuramitsu, Ryoji Masui
Role of RecJ-like protein with 5'-3' exonuclease activity in oligo(deoxy)nucleotide degradation.
J Biol Chem: 2011, 286(4);2807-16
[PubMed:21087930] [WorldCat.org] [DOI] (I p)

Ming Fang, Wencke-Maria Zeisberg, Ciaran Condon, Vasily Ogryzko, Antoine Danchin, Undine Mechold
Degradation of nanoRNA is performed by multiple redundant RNases in Bacillus subtilis.
Nucleic Acids Res: 2009, 37(15);5114-25
[PubMed:19553197] [WorldCat.org] [DOI] (I p)

Jiaqin Zhang, Indranil Biswas
3'-Phosphoadenosine-5'-phosphate phosphatase activity is required for superoxide stress tolerance in Streptococcus mutans.
J Bacteriol: 2009, 191(13);4330-40
[PubMed:19429620] [WorldCat.org] [DOI] (I p)

Undine Mechold, Gang Fang, Saravuth Ngo, Vasily Ogryzko, Antoine Danchin
YtqI from Bacillus subtilis has both oligoribonuclease and pAp-phosphatase activity.
Nucleic Acids Res: 2007, 35(13);4552-61
[PubMed:17586819] [WorldCat.org] [DOI] (I p)

Jean-Christophe Meile, Ling Juan Wu, S Dusko Ehrlich, Jeff Errington, Philippe Noirot
Systematic localisation of proteins fused to the green fluorescent protein in Bacillus subtilis: identification of new proteins at the DNA replication factory.
Proteomics: 2006, 6(7);2135-46
[PubMed:16479537] [WorldCat.org] [DOI] (P p)

Alia Lapidus, Nathalie Galleron, Alexei Sorokin, S Dusko Ehrlich
Sequencing and functional annotation of the Bacillus subtilis genes in the 200 kb rrnB-dnaB region.
Microbiology (Reading): 1997, 143 ( Pt 11);3431-3441
[PubMed:9387221] [WorldCat.org] [DOI] (P p)