MetI

• Description: O-succinylhomoserine lyase (L-cysteine, H2S, methanethiol, elimination)
  
  

Gene name	metI
Synonyms	yjcI
Essential	no
Product	O-succinylhomoserine lyase (L-cysteine, H2S, methanethiol, elimination)
Function	biosynthesis of methionine
Gene expression levels in SubtiExpress: metI
Metabolic function and regulation of this protein in SubtiPathways: metI
MW, pI	41 kDa, 5.019
Gene length, protein length	1119 bp, 373 aa
Immediate neighbours	yjcH, metC
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis/ acquisition of amino acids

This gene is a member of the following regulons

S-box

The gene

Basic information

• Locus tag: BSU11870

Database entries

• BsubCyc: BSU11870

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU11870

• Structure:

• UniProt: O31631

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: metI-metC PubMed

• Expression browser: metI PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed by casamino acids PubMed
  • induced by methionine starvation (S-box) PubMed

• Regulatory mechanism: S-box: transcription termination/ antitermination, the S-box riboswitch binds S-adenosylmethionine resulting in termination PubMed

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 1079 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 949 PubMed

Biological materials

• Mutant:
  • 1A941 ( metI::spec), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: