KatA

• Description: main vegetative catalase 1
  
  

Gene name	katA
Synonyms	kat-19
Essential	no
Product	vegetative catalase
Function	detoxification (degradation) of hydrogen peroxide
Gene expression levels in SubtiExpress: katA
Metabolic function and regulation of this protein in SubtiPathways: katA
MW, pI	54 kDa, 6.151
Gene length, protein length	1449 bp, 483 aa
Immediate neighbours	senS, ssuB
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

resistance against oxidative and electrophile stress, phosphoproteins

This gene is a member of the following regulons

PerR regulon

The gene

Basic information

• Locus tag: BSU08820

Phenotypes of a mutant

• increased sensitivity to oxidative stress PubMed

Database entries

• BsubCyc: BSU08820

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: 2 H₂O₂ = O₂ + 2 H₂O (according to Swiss-Prot)

• Protein family: catalase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:
  • phosphorylated on Arg-366 PubMed

• Cofactor(s): contains an iron-sulfur cluster, heme

• Effectors of protein activity:

• Interactions:

• Localization:
  • cytoplasm (according to Swiss-Prot)

Database entries

• BsubCyc: BSU08820

• Structure: 1SI8 (enzyme from Enterococcus faecalis, 68% identity)

• UniProt: P26901

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: katA PubMed

• Expression browser: katA PubMed

• Sigma factor:

• Regulation:
  • induced in the presence of hydrogen peroxide and paraquat (PerR) PubMed

• Regulatory mechanism:
  • PerR: transcription repression PubMed

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 1347 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 452 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: