LytC

• Description: N-acetylmuramoyl-L-alanine amidase, required for flagellar function PubMed
  
  

Gene name	lytC
Synonyms	cwlB
Essential	no
Product	N-acetylmuramoyl-L-alanine amidase
Function	major autolysin, cell separation, wall turnover lysis, motility and general cell lysis induced by sodium azide
Gene expression levels in SubtiExpress: lytC lysis, motility and general cell lysis induced by sodium azide
Metabolic function and regulation of this protein in SubtiPathways: LytC
MW, pI	52 kDa, 10.108
Gene length, protein length	1488 bp, 496 aa
Immediate neighbours	tuaA, lytB
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

cell wall degradation/ turnover, motility and chemotaxis

This gene is a member of the following regulons

SigD regulon, SlrR regulon, YvrHb regulon

The gene

Basic information

• Locus tag: BSU35620

Phenotypes of a mutant

• impaired in motility, the phenotype is suppressed by mutations in lonA or smiA PubMed

Database entries

• BsubCyc: BSU35620

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides (according to Swiss-Prot)

• Protein family: N-acetylmuramoyl-L-alanine amidase 3 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains: contains an amidase_3 domain (like CwlC, CwlD, YqiI, YrvJ)

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • secreted (according to Swiss-Prot)

Database entries

• BsubCyc: BSU35620

• Structure:

• UniProt: Q02114

• KEGG entry: [3]

• E.C. number: 3.5.1.28

Expression and regulation

• Operon: lytA-lytB-lytC PubMed

• Expression browser: lytC PubMed

• Sigma factor: SigA PubMed, SigD PubMed

• Regulation:

• Regulatory mechanism:
  • YvrHb: transcription repression PubMed
  • SlrR (in complex with SinR: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:
  • 1A789 ( lytC::kan), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: