SppA

• Description: signal peptide peptidase required for efficient processing of pre-proteins, cleaves remnant signal peptides within the cellular membrane
  
  

Gene name	sppA
Synonyms	yteI
Essential	no
Product	signal peptide peptidase
Function	protein secretion
Gene expression levels in SubtiExpress: sppA
Metabolic function and regulation of this protein in SubtiPathways: SppA
MW, pI	36 kDa, 7.314
Gene length, protein length	1005 bp, 335 aa
Immediate neighbours	yteJ, ytdI
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

protein secretion, cell envelope stress proteins (controlled by SigM, V, W, X, Y), membrane proteins

This gene is a member of the following regulons

SigW regulon

The gene

Basic information

• Locus tag: BSU29530

Phenotypes of a mutant

• more sensitive to nisin PubMed

Database entries

• BsubCyc: BSU29530

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • serine protease that functions to cleave the remnant signal peptides left behind after protein secretion and cleavage by signal peptidases

• Protein family: peptidase S49 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:
  • the protein forms a dome shaped octameric complex PubMed

• Localization:
  • cell membrane PubMed

Database entries

• BsubCyc: BSU29530

• Structure: 3RST PubMed

• UniProt: O34525

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: sppA-yteJ PubMed

• Expression browser: sppA PubMed

• Sigma factor: SigW PubMed

• Regulation:
  • induced in response to cell wall stress (SigW) PubMed

• Regulatory mechanism:

• Additional information:
  • self-processes its own C-termini PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: