UvrA

• Description: excinuclease ABC (subunit A)
  
  

Gene name	uvrA
Synonyms
Essential	no
Product	excinuclease ABC (subunit A)
Function	DNA repair after UV damage
Gene expression levels in SubtiExpress: uvrA
Interactions involving this protein in SubtInteract: UvrA
MW, pI	105 kDa, 5.843
Gene length, protein length	2871 bp, 957 aa
Immediate neighbours	yvzB, uvrB
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

DNA repair/ recombination

This gene is a member of the following regulons

LexA regulon

The gene

Basic information

• Locus tag: BSU35160

Database entries

• BsubCyc: BSU35160

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: UvrA subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • Mfd-UvrA PubMed
  • UvrA-UvrB PubMed

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• BsubCyc: BSU35160

• Structure: 2R6F (Geobacillus stearothermophilus)

• UniProt: O34863

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: uvrB-uvrA (according to DBTBS)

• Expression browser: uvrA PubMed

• Sigma factor:

• Regulation:
  • induced upon DNA damage (LexA) PubMed

• Regulatory mechanism:
  • LexA: transcription repression PubMed

• Additional information:
  • during sporulation expressed both in the forespore and the mother cell PubMed

Biological materials

• mutant: GP1175 (del uvrAB::ermC) (available in the Stülke lab)

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Original publications

Fernando H Ramírez-Guadiana, Rocío Del Carmen Barajas-Ornelas, Víctor M Ayala-García, Ronald E Yasbin, Eduardo Robleto, Mario Pedraza-Reyes
Transcriptional coupling of DNA repair in sporulating Bacillus subtilis cells.
Mol Microbiol: 2013, 90(5);1088-99
[PubMed:24118570] [WorldCat.org] [DOI] (I p)

Laura Manelyte, Young-In T Kim, Abigail J Smith, Rachel M Smith, Nigel J Savery
Regulation and rate enhancement during transcription-coupled DNA repair.
Mol Cell: 2010, 40(5);714-24
[PubMed:21145481] [WorldCat.org] [DOI] (I p)

Bradley T Smith, Alan D Grossman, Graham C Walker
Localization of UvrA and effect of DNA damage on the chromosome of Bacillus subtilis.
J Bacteriol: 2002, 184(2);488-93
[PubMed:11751826] [WorldCat.org] [DOI] (P p)

C M Lovett, K C Cho, T M O'Gara
Purification of an SOS repressor from Bacillus subtilis.
J Bacteriol: 1993, 175(21);6842-9
[PubMed:8226626] [WorldCat.org] [DOI] (P p)