MutTA

• Description: error prevention oxidized guanine system, confers protection against oxidative stress to vegetative cells, moreover the protein has RNA pyrophosphohydrolase activity
  
  

Gene name	mutTA
Synonyms	ytkD, rppH
Essential	no
Product	8-oxo-dGTPase (antimutator), RNA pyrophosphohydrolase
Function	DNA repair, RNA degradation
Gene expression levels in SubtiExpress: mutTA
MW, pI	18 kDa, 5.952
Gene length, protein length	474 bp, 158 aa
Immediate neighbours	ytlD, ytkC
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

DNA repair/ recombination, sporulation proteins, RNases

This gene is a member of the following regulons

SigF regulon

The gene

Basic information

• Locus tag: BSU30630

Database entries

• BsubCyc: BSU30630

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • removal of pyrophosphate from the 5' end of mRNAs to make the RNA accessible for degradation by RNases PubMed
  • RppH requires at least two unpaired nucleotides at the 5' end of its RNA substrates and prefers three or more. The second of these 5'-terminal nucleotides must be G, whereas a less strict preference for a purine is evident at the third position, and A is slightly favored over G at the first position PubMed

• Protein family: Nudix hydrolase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU30630

• Structure: 4JZV PubMed

• UniProt: O35013

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: mutTA PubMed

• Expression browser: mutTA PubMed

• Sigma factor: SigA PubMed, SigF PubMed

• Regulation:
  • expressed during logarithmic growth (SigA) and early during sporulation in the forespore (SigF) PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Martin Lehnik-Habrink, Richard J Lewis, Ulrike Mäder, Jörg Stülke
RNA degradation in Bacillus subtilis: an interplay of essential endo- and exoribonucleases.
Mol Microbiol: 2012, 84(6);1005-17
[PubMed:22568516] [WorldCat.org] [DOI] (I p)

Original publications

Ping-kun Hsieh, Jamie Richards, Quansheng Liu, Joel G Belasco
Specificity of RppH-dependent RNA degradation in Bacillus subtilis.
Proc Natl Acad Sci U S A: 2013, 110(22);8864-9
[PubMed:23610425] [WorldCat.org] [DOI] (I p)

Jérémie Piton, Valéry Larue, Yann Thillier, Audrey Dorléans, Olivier Pellegrini, Inés Li de la Sierra-Gallay, Jean-Jacques Vasseur, Françoise Debart, Carine Tisné, Ciarán Condon
Bacillus subtilis RNA deprotection enzyme RppH recognizes guanosine in the second position of its substrates.
Proc Natl Acad Sci U S A: 2013, 110(22);8858-63
[PubMed:23610407] [WorldCat.org] [DOI] (I p)

Jamie Richards, Quansheng Liu, Olivier Pellegrini, Helena Celesnik, Shiyi Yao, David H Bechhofer, Ciarán Condon, Joel G Belasco
An RNA pyrophosphohydrolase triggers 5'-exonucleolytic degradation of mRNA in Bacillus subtilis.
Mol Cell: 2011, 43(6);940-9
[PubMed:21925382] [WorldCat.org] [DOI] (I p)

Luz E Vidales, Lluvia C Cárdenas, Eduardo Robleto, Ronald E Yasbin, Mario Pedraza-Reyes
Defects in the error prevention oxidized guanine system potentiate stationary-phase mutagenesis in Bacillus subtilis.
J Bacteriol: 2009, 191(2);506-13
[PubMed:19011023] [WorldCat.org] [DOI] (I p)

Francisco X Castellanos-Juárez, Carlos Alvarez-Alvarez, Ronald E Yasbin, Barbara Setlow, Peter Setlow, Mario Pedraza-Reyes
YtkD and MutT protect vegetative cells but not spores of Bacillus subtilis from oxidative stress.
J Bacteriol: 2006, 188(6);2285-9
[PubMed:16513759] [WorldCat.org] [DOI] (P p)

Stephanie T Wang, Barbara Setlow, Erin M Conlon, Jessica L Lyon, Daisuke Imamura, Tsutomu Sato, Peter Setlow, Richard Losick, Patrick Eichenberger
The forespore line of gene expression in Bacillus subtilis.
J Mol Biol: 2006, 358(1);16-37
[PubMed:16497325] [WorldCat.org] [DOI] (P p)

Wenlian Xu, Candice R Jones, Christopher A Dunn, Maurice J Bessman
Gene ytkD of Bacillus subtilis encodes an atypical nucleoside triphosphatase member of the Nudix hydrolase superfamily.
J Bacteriol: 2004, 186(24);8380-4
[PubMed:15576788] [WorldCat.org] [DOI] (P p)

Martha I Ramírez, Francisco X Castellanos-Juárez, Ronald E Yasbin, Mario Pedraza-Reyes
The ytkD (mutTA) gene of Bacillus subtilis encodes a functional antimutator 8-Oxo-(dGTP/GTP)ase and is under dual control of sigma A and sigma F RNA polymerases.
J Bacteriol: 2004, 186(4);1050-9
[PubMed:14761999] [WorldCat.org] [DOI] (P p)

Alia Lapidus, Nathalie Galleron, Alexei Sorokin, S Dusko Ehrlich
Sequencing and functional annotation of the Bacillus subtilis genes in the 200 kb rrnB-dnaB region.
Microbiology (Reading): 1997, 143 ( Pt 11);3431-3441
[PubMed:9387221] [WorldCat.org] [DOI] (P p)