ytlD

168

similar to ABC transporter (permease)

Locus: BSU_30620

Molecular weight: 30.15 kDa

Isoelectric point: 9.77

Protein length: 270 aa

Gene length: 813 bp

Function: unknown

Product: ABC transporter (permease)

Essential: no

Synonyms: ytlD

Genomic Context

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List of homologs in different organisms, belongs to COG0600 (Galperin et al., 2021)

Gene

Coordinates: 3,134,144  3,134,956

The protein

Protein family

• Binding-protein-dependent transport system permease family (according to UniProt)

Domains

• ABC transmembrane type-1 domain (aa 74-255) (according to UniProt)

Structure

• O34649 (AlphaFold)

Localization

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• Genes: ytlA-ytlC-ytlD

  Description: PubMed

  Regulation

  • expressed late during sporulation in the mother cell (SigK) PubMed

  Sigma factors

  • SigK: sigma factor, PubMed, in sigK regulon

Biological materials

Mutant

• MGNA-A298 (ytlD::erm), available at the NBRP B. subtilis, Japan
• BKE30620 (ytlD::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GAATTGATACTGCTCGTGAA,  downstream forward: _UP4_TAAAAAACCCGGCACATGTG
• BKK30620 (ytlD::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GAATTGATACTGCTCGTGAA,  downstream forward: _UP4_TAAAAAACCCGGCACATGTG

References

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