TrmB

• Description: tRNA (m7G46) methyltransferase
  
  

Gene name	trmB
Synonyms	ytmQ
Essential	no
Product	tRNA (m7G46) methyltransferase
Function	tRNA modification
Gene expression levels in SubtiExpress: trmB
MW, pI	24 kDa, 6.351
Gene length, protein length	639 bp, 213 aa
Immediate neighbours	ytnP, ytzH
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

translation

The gene

Basic information

• Locus tag: BSU29900

Database entries

• BsubCyc: BSU29900

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: S-adenosyl-L-methionine + tRNA = S-adenosyl-L-homocysteine + tRNA containing N(7)-methylguanine (according to Swiss-Prot)

• Protein family: TrmB family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU29900

• Structure: 2FCA

• UniProt: O34522

• KEGG entry: [3]

• E.C. number: 2.1.1.33

Expression and regulation

• Operon: trmB PubMed

• Expression browser: trmB PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Ingrid Zegers, Daniel Gigot, Françoise van Vliet, Catherine Tricot, Stéphane Aymerich, Janusz M Bujnicki, Jan Kosinski, Louis Droogmans
Crystal structure of Bacillus subtilis TrmB, the tRNA (m7G46) methyltransferase.
Nucleic Acids Res: 2006, 34(6);1925-34
[PubMed:16600901] [WorldCat.org] [DOI] (I e)

Alia Lapidus, Nathalie Galleron, Alexei Sorokin, S Dusko Ehrlich
Sequencing and functional annotation of the Bacillus subtilis genes in the 200 kb rrnB-dnaB region.
Microbiology (Reading): 1997, 143 ( Pt 11);3431-3441
[PubMed:9387221] [WorldCat.org] [DOI] (P p)