PstBA

• Description: phosphate ABC transporter (ATP-binding protein)
  
  

Gene name	pstBA
Synonyms	pstB, yqgJ, yzmE
Essential	no
Product	phosphate ABC transporter (ATP-binding protein)
Function	high-affinity phosphate uptake
Gene expression levels in SubtiExpress: pstBA
Interactions involving this protein in SubtInteract: PstBA
MW, pI	29 kDa, 7.897
Gene length, protein length	807 bp, 269 aa
Immediate neighbours	pstBB, pstA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

ABC transporters, phosphate metabolism, membrane proteins

This gene is a member of the following regulons

PhoP regulon

The gene

Basic information

• Locus tag: BSU24960

Database entries

• BsubCyc: BSU24960

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + H₂O + phosphate(Out) = ADP + phosphate + phosphate(In) (according to Swiss-Prot)

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • (PstBA-PstBB)-(PstA-PstC)-PstS PubMed

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• BsubCyc: BSU24960

• Structure:

• UniProt: P46341

• KEGG entry: [2]

• E.C. number: 3.6.3.27

Expression and regulation

• Operon: pstS-pstC-pstA-pstBA-pstBB PubMed

• Expression browser: pstBA PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • expressed under conditions of phosphate limitation (5000-fold induced) (PhoP) PubMed

• Regulatory mechanism:
  • PhoP-P: transcription activation PubMed

• Additional information:
  • mRNA processing between pstS and pstC to maintain higher concentrations of PstS relative to other components of the ABC transporter PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Marion Hulett, University of Illinois at Chicago, USA Homepage

References

Nicholas E E Allenby, Nicola O'Connor, Zoltán Prágai, Noel M Carter, Marcus Miethke, Susanne Engelmann, Michael Hecker, Anil Wipat, Alan C Ward, Colin R Harwood
Post-transcriptional regulation of the Bacillus subtilis pst operon encoding a phosphate-specific ABC transporter.
Microbiology (Reading): 2004, 150(Pt 8);2619-2628
[PubMed:15289558] [WorldCat.org] [DOI] (P p)

Akram Atalla, Wolfgang Schumann
The pst operon of Bacillus subtilis is specifically induced by alkali stress.
J Bacteriol: 2003, 185(16);5019-22
[PubMed:12897025] [WorldCat.org] [DOI] (P p)

H Antelmann, C Scharf, M Hecker
Phosphate starvation-inducible proteins of Bacillus subtilis: proteomics and transcriptional analysis.
J Bacteriol: 2000, 182(16);4478-90
[PubMed:10913081] [WorldCat.org] [DOI] (P p)

Y Quentin, G Fichant, F Denizot
Inventory, assembly and analysis of Bacillus subtilis ABC transport systems.
J Mol Biol: 1999, 287(3);467-84
[PubMed:10092453] [WorldCat.org] [DOI] (P p)

W Liu, Y Qi, F M Hulett
Sites internal to the coding regions of phoA and pstS bind PhoP and are required for full promoter activity.
Mol Microbiol: 1998, 28(1);119-30
[PubMed:9593301] [WorldCat.org] [DOI] (P p)

Y Qi, Y Kobayashi, F M Hulett
The pst operon of Bacillus subtilis has a phosphate-regulated promoter and is involved in phosphate transport but not in regulation of the pho regulon.
J Bacteriol: 1997, 179(8);2534-9
[PubMed:9098050] [WorldCat.org] [DOI] (P p)