YosS

• Description: dUTP diphosphatase
  
  

Gene name	yosS
Synonyms
Essential	no
Product	dUTP diphosphatase
Function	nucleotide metabolism
Gene expression levels in SubtiExpress: yosS
MW, pI	16 kDa, 7.086
Gene length, protein length	426 bp, 142 aa
Immediate neighbours	yosT, yosR
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

nucleotide metabolism/ other, SP-beta prophage

The gene

Basic information

• Locus tag: BSU20020

Database entries

• BsubCyc: BSU20020

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s): YncF

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • forms homotrimers PubMed

• Localization:

Database entries

• BsubCyc: BSU20020

• Structure: 2BAZ PubMed

• UniProt: O34919

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Expression browser: yosS PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Javier García-Nafría, Jennifer Timm, Charlotte Harrison, Johan P Turkenburg, Keith S Wilson
Tying down the arm in Bacillus dUTPase: structure and mechanism.
Acta Crystallogr D Biol Crystallogr: 2013, 69(Pt 8);1367-80
[PubMed:23897460] [WorldCat.org] [DOI] (I p)

Pierre Nicolas, Ulrike Mäder, Etienne Dervyn, Tatiana Rochat, Aurélie Leduc, Nathalie Pigeonneau, Elena Bidnenko, Elodie Marchadier, Mark Hoebeke, Stéphane Aymerich, Dörte Becher, Paola Bisicchia, Eric Botella, Olivier Delumeau, Geoff Doherty, Emma L Denham, Mark J Fogg, Vincent Fromion, Anne Goelzer, Annette Hansen, Elisabeth Härtig, Colin R Harwood, Georg Homuth, Hanne Jarmer, Matthieu Jules, Edda Klipp, Ludovic Le Chat, François Lecointe, Peter Lewis, Wolfram Liebermeister, Anika March, Ruben A T Mars, Priyanka Nannapaneni, David Noone, Susanne Pohl, Bernd Rinn, Frank Rügheimer, Praveen K Sappa, Franck Samson, Marc Schaffer, Benno Schwikowski, Leif Steil, Jörg Stülke, Thomas Wiegert, Kevin M Devine, Anthony J Wilkinson, Jan Maarten van Dijl, Michael Hecker, Uwe Völker, Philippe Bessières, Philippe Noirot
Condition-dependent transcriptome reveals high-level regulatory architecture in Bacillus subtilis.
Science: 2012, 335(6072);1103-6
[PubMed:22383849] [WorldCat.org] [DOI] (I p)

Javier García-Nafría, Maria Harkiolaki, Rebecca Persson, Mark J Fogg, Keith S Wilson
The structure of Bacillus subtilis SPβ prophage dUTPase and its complexes with two nucleotides.
Acta Crystallogr D Biol Crystallogr: 2011, 67(Pt 3);167-75
[PubMed:21358047] [WorldCat.org] [DOI] (I p)

Gui Lan Li, Juan Wang, Lan Fen Li, Xiao Dong Su
Crystallization and preliminary X-ray analysis of three dUTPases from Gram-positive bacteria.
Acta Crystallogr Sect F Struct Biol Cryst Commun: 2009, 65(Pt 4);339-42
[PubMed:19342774] [WorldCat.org] [DOI] (I p)