IspC

• Description: 1-deoxy-D-xylulose-5-phosphate reductoisomerase, second step in the MEP pathway of isoprenoid biosynthesis
  
  

Gene name	ispC
Synonyms	yluB, dxr
Essential	yes PubMed
Product	1-deoxy-D-xylulose-5-phosphate reductoisomerase
Function	MEP pathway of isoprenoid biosynthesis
Gene expression levels in SubtiExpress: ispC
MW, pI	43 kDa, 5.801
Gene length, protein length	1164 bp, 388 aa
Immediate neighbours	cdsA, rasP
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis of lipids, essential genes

The gene

Basic information

• Locus tag: BSU16550

Phenotypes of a mutant

essential PubMed

Database entries

• BsubCyc: BSU16550

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: 2-C-methyl-D-erythritol 4-phosphate + NADP⁺ = 1-deoxy-D-xylulose 5-phosphate + NADPH (according to Swiss-Prot)

• Protein family: DXR family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU16550

• Structure: 1R0K (from Zymomonas mobilis, 45% identity) PubMed

• UniProt: O31753

• KEGG entry: [2]

• E.C. number: 1.1.1.267

Expression and regulation

• Operon:

• Expression browser: ispC PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Becky M Hess, Junfeng Xue, Lye Meng Markillie, Ronald C Taylor, H Steven Wiley, Birgitte K Ahring, Bryan Linggi
Coregulation of Terpenoid Pathway Genes and Prediction of Isoprene Production in Bacillus subtilis Using Transcriptomics.
PLoS One: 2013, 8(6);e66104
[PubMed:23840410] [WorldCat.org] [DOI] (I e)

Junfeng Xue, Birgitte K Ahring
Enhancing isoprene production by genetic modification of the 1-deoxy-d-xylulose-5-phosphate pathway in Bacillus subtilis.
Appl Environ Microbiol: 2011, 77(7);2399-405
[PubMed:21296950] [WorldCat.org] [DOI] (I p)

Mattijs K Julsing, Michael Rijpkema, Herman J Woerdenbag, Wim J Quax, Oliver Kayser
Functional analysis of genes involved in the biosynthesis of isoprene in Bacillus subtilis.
Appl Microbiol Biotechnol: 2007, 75(6);1377-84
[PubMed:17458547] [WorldCat.org] [DOI] (P p)

Stefano Ricagno, Sigrid Grolle, Stephanie Bringer-Meyer, Hermann Sahm, Ylva Lindqvist, Gunter Schneider
Crystal structure of 1-deoxy-d-xylulose-5-phosphate reductoisomerase from Zymomonas mobilis at 1.9-A resolution.
Biochim Biophys Acta: 2004, 1698(1);37-44
[PubMed:15063313] [WorldCat.org] [DOI] (P p)