SpoIISB

• Description: disruption blocks sporulation after septum formation
  
  

Gene name	spoIISB
Synonyms
Essential	no
Product	anti-toxin
Function	sporulation
Gene expression levels in SubtiExpress: spoIISB
Interactions involving this protein in SubtInteract: SpoIISB
MW, pI	6 kDa, 10.794
Gene length, protein length	168 bp, 56 aa
Immediate neighbours	xlyA, spoIISA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

sporulation/ other, toxins, antitoxins and immunity against toxins

The gene

Basic information

• Locus tag: BSU12820

Phenotypes of a mutant

• sporulation efficiency is reduced by four orders of magnitude PubMed

Database entries

• BsubCyc: BSU12820

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • SpoIISA-SpoIISB PubMed

• Localization:

Database entries

• BsubCyc: BSU12820

• Structure: 3O6Q (cytoplasmic fragment of SpoIISA (CSpoIISA) in complex with SpoIISB) PubMed

• UniProt: O34800

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:
• spoIISA-spoIISB PubMed
• spoIISB PubMed

• Expression browser: spoIISB PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:
  • 1S124 ( spoIISB::tet), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Patrik Florek, Vladimir M Levdikov, Elena Blagova, Andrey A Lebedev, Rostislav Škrabana, Stanislava Resetárová, Pamela Pavelcíková, Imrich Barak, Anthony J Wilkinson
The structure and interactions of SpoIISA and SpoIISB, a toxin-antitoxin system in Bacillus subtilis.
J Biol Chem: 2011, 286(8);6808-19
[PubMed:21147767] [WorldCat.org] [DOI] (I p)

Patrik Florek, Katarína Muchová, Pamela Pavelcíková, Imrich Barák
Expression of functional Bacillus SpoIISAB toxin-antitoxin modules in Escherichia coli.
FEMS Microbiol Lett: 2008, 278(2);177-84
[PubMed:18096016] [WorldCat.org] [DOI] (P p)

E Adler, I Barák, P Stragier
Bacillus subtilis locus encoding a killer protein and its antidote.
J Bacteriol: 2001, 183(12);3574-81
[PubMed:11371520] [WorldCat.org] [DOI] (P p)