TrpS

• Description: tryptophanyl-tRNA synthetase
  
  

Gene name	trpS
Synonyms
Essential	yes PubMed
Product	tryptophanyl-tRNA synthetase
Function	translation
Gene expression levels in SubtiExpress: trpS
Metabolic function and regulation of this protein in SubtiPathways: trpS
MW, pI	37 kDa, 6.542
Gene length, protein length	990 bp, 330 aa
Immediate neighbours	yjbA, oppA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

translation, essential genes

This gene is a member of the following regulons

T-box

The gene

Basic information

• Locus tag: BSU11420

Phenotypes of a mutant

essential PubMed

Database entries

• BsubCyc: BSU11420

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp) (according to Swiss-Prot)

• Protein family: class-I aminoacyl-tRNA synthetase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• BsubCyc: BSU11420

• Structure:
  • 3PRH (Val144Pro mutant) PubMed
  • 1BM2 (complex with tryptophan, Geobacillus stearothermophilus)
  • 1MAW (complex with ATP, Geobacillus stearothermophilus)

• UniProt: P21656

• KEGG entry: [3]

• E.C. number: 6.1.1.2

Expression and regulation

• Operon: trpS PubMed

• Expression browser: trpS PubMed

• Sigma factor:

• Regulation:
  • induced by tryptophan limitation (T-box) PubMed

• Regulatory mechanism:
  • T-box: transcriptional antitermination in the absence of tryptophan (upon tRNA-binding to the mRNA leader region) PubMed

• Additional information:
  • A ncRNA is predicted between trpS and oppA PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: