CarB

• Description: carbamoyl-phosphate transferase-arginine subunit B)
  
  

Gene name	carB
Synonyms	cpa
Essential	no
Product	carbamoyl-phosphate transferase-arginine subunit B)
Function	biosynthesis of arginine
Gene expression levels in SubtiExpress: carB
Interactions involving this protein in SubtInteract: CarB
Metabolic function and regulation of this protein in SubtiPathways: carB
MW, pI	112 kDa, 4.712
Gene length, protein length	3090 bp, 1030 aa
Immediate neighbours	carA, argF
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis/ acquisition of amino acids, most abundant proteins

This gene is a member of the following regulons

AhrC regulon

The gene

Basic information

• Locus tag: BSU11240

Database entries

• BsubCyc: BSU11240

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: 2 ATP + L-glutamine + HCO₃^- + H₂O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate (according to Swiss-Prot)

• Protein family: carB family (according to Swiss-Prot)

• Paralogous protein(s): PyrAB

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions: CarA-CarB

• Localization:

Database entries

• BsubCyc: BSU11240

• Structure: 1JDB (from Escherichia coli, 47% identity, 64% similarity) PubMed

• UniProt: P18185

• KEGG entry: [3]

• E.C. number: 6.3.5.5

Additional information

• subject to Clp-dependent proteolysis upon glucose starvation PubMed

Expression and regulation

• Operon: argC-argJ-argB-argD-carA-carB-argF PubMed

• Expression browser: carB PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed by casamino acids PubMed
  • repressed by arginine (AhrC) PubMed

• Regulatory mechanism:
  • AhrC: transcription repression PubMed

• Additional information:
  • subject to Clp-dependent proteolysis upon glucose starvation PubMed
  • belongs to the 100 most abundant proteins PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Christine Eymann, Annette Dreisbach, Dirk Albrecht, Jörg Bernhardt, Dörte Becher, Sandy Gentner, Le Thi Tam, Knut Büttner, Gerrit Buurman, Christian Scharf, Simone Venz, Uwe Völker, Michael Hecker
A comprehensive proteome map of growing Bacillus subtilis cells.
Proteomics: 2004, 4(10);2849-76
[PubMed:15378759] [WorldCat.org] [DOI] (P p)

Ulrike Mäder, Georg Homuth, Christian Scharf, Knut Büttner, Rüdiger Bode, Michael Hecker
Transcriptome and proteome analysis of Bacillus subtilis gene expression modulated by amino acid availability.
J Bacteriol: 2002, 184(15);4288-95
[PubMed:12107147] [WorldCat.org] [DOI] (P p)

A Mountain, N H Mann, R N Munton, S Baumberg
Cloning of a Bacillus subtilis restriction fragment complementing auxotrophic mutants of eight Escherichia coli genes of arginine biosynthesis.
Mol Gen Genet: 1984, 197(1);82-9
[PubMed:6096675] [WorldCat.org] [DOI] (P p)