ecsB

168

ABC transporter ( membrane protein), mutation affects proteolysis by RasP (no processing of RsgI resulting in absence if SigI activity)

Locus: BSU_10050

Molecular weight: 47.14 kDa

Isoelectric point: 10.18

Protein length: 408 aa

Gene length: 1227 bp

Function: regulation of the secretion apparatus and of intra-membrane proteolysis

Product: ABC transporter ( membrane protein)

Essential: no

Synonyms: ecsB, prsT, yhaC

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG4473 (Galperin et al., 2021)

Gene

Coordinates: 1,078,176  1,079,402

Phenotypes of a mutant

• inactivation of ''ecsB'' reduces sporulation efficiency to 13.4% that of wild type cells PubMed
• no processing of RsiW and FtsL, impaired activity of SigW PubMed
• a cwlO ecsB mutant is not viable, due to lack of expression of lytE PubMed

The protein

Structure

• P55340 (AlphaFold)

Localization

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• Genes: ecsA-ecsB-ecsC

  Description: PubMed

Additional information

• the mRNA is very stable (> 15 min) PubMed

Biological materials

Mutant

• BKE10050 (ecsB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CCGCGACTGCCAAATATCAA,  downstream forward: _UP4_CTGTGAACTGAAAAGAGGTA
• BKK10050 (ecsB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CCGCGACTGCCAAATATCAA,  downstream forward: _UP4_CTGTGAACTGAAAAGAGGTA

References

Reviews

Loading

Original Publications

Loading