yhbJ

168

putative efflux system component

Locus: BSU_09000

Molecular weight: 23.47 kDa

Isoelectric point: 4.37

Protein length: 221 aa

Gene length: 666 bp

Function: unknown

Product: unknown

Essential: no

Synonyms: yhbJ

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG1566 (Galperin et al., 2021)

Gene

Coordinates: 977,069  977,734

The protein

Protein family

• Membrane fusion protein (MFP) (TC 8.A.1) family (according to UniProt)

Structure

• 4TKO (PDB) (from Aquifex aeolicus, corresponds to aa 74 ... 202, 35% identity) PubMed
• O31593 (AlphaFold)

Localization

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• Genes: yhbI-yhbJ-yhcA-yhcB-yhcC

  Description: PubMed

  Additional information

  • the mRNA is substantially stabilized upon depletion of RNase Y (the half-life of the mRNA increases from 4 to 41 min) PubMed

Biological materials

Mutant

• MGNA-B470 (yhbJ::erm), available at the NBRP B. subtilis, Japan
• BKE09000 (yhbJ::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTTGCGGAAAGCTGGTG,  downstream forward: _UP4_TAAAACAGATCAGTCATCAA
• BKK09000 (yhbJ::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTTGCGGAAAGCTGGTG,  downstream forward: _UP4_TAAAACAGATCAGTCATCAA

References

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