citT

168

two-component response regulator, regulation of citrate uptake

Locus: BSU_07590

Molecular weight: 25.26 kDa

Isoelectric point: 6.36

Protein length: 226 aa

Gene length: 681 bp

Function: regulation of citrate uptake

Product: two-component response regulator

Essential: no

Synonyms: citT, yflQ

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG4565 (Galperin et al., 2021)

Gene

Coordinates: 832,545  833,225

Phenotypes of a mutant

• no growth with citrate as sole carbon source PubMed

The protein

Catalyzed reaction/ biological activity

• binding to the promoter of the ''citM-yflN'' operon and activation of transcription in the presence of citrate PubMed

Domains

• Response regulatory domain (aa 3-119) (according to UniProt)

Structure

• 1YIO (PDB) (from Pseudomonas fluorescens, corresponds to aa 50 ... 207, 22% identity) PubMed
• O34534 (AlphaFold)

Modification

• phosphorylated by CitS on an Asp residue

Effectors of protein activity

• phosphorylation likely affects DNA-binding activity

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: citS-citT-yflP

  Description: PubMed

Biological materials

Mutant

• MGNA-C250 (citT::erm), available at the NBRP B. subtilis, Japan
• BKE07590 (citT::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_AATCATCCTCCGCAATCGCG,  downstream forward: _UP4_TATTATTTGGCGGCGGATTA
• BKK07590 (citT::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_AATCATCCTCCGCAATCGCG,  downstream forward: _UP4_TATTATTTGGCGGCGGATTA

References

Reviews

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Original Publications

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