YdaP
- Description: general stress protein, required for survival of ethanol stress, putative pyruvate oxidase
Gene name | ydaP |
Synonyms | |
Essential | no |
Product | putative pyruvate oxidase |
Function | unknown |
Gene expression levels in SubtiExpress: ydaP | |
MW, pI | 62 kDa, 5.217 |
Gene length, protein length | 1722 bp, 574 aa |
Immediate neighbours | mutT, ydzK |
Sequences | Protein DNA DNA_with_flanks |
Genetic context This image was kindly provided by SubtiList
| |
Expression at a glance PubMed |
Contents
Categories containing this gene/protein
general stress proteins (controlled by SigB), poorly characterized/ putative enzymes
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU04340
Phenotypes of a mutant
Database entries
- BsubCyc: BSU04340
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family:
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
Database entries
- BsubCyc: BSU04340
- UniProt: P96591
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Operon: ydaP PubMed
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant: GP457 (spc), available in Stülke lab
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Danilo Meyer, Piotr Neumann, Ralf Ficner, Kai Tittmann
Observation of a stable carbene at the active site of a thiamin enzyme.
Nat Chem Biol: 2013, 9(8);488-90
[PubMed:23748673]
[WorldCat.org]
[DOI]
(I p)
Danilo Meyer, Piotr Neumann, Eline Koers, Hanno Sjuts, Stefan Lüdtke, George M Sheldrick, Ralf Ficner, Kai Tittmann
Unexpected tautomeric equilibria of the carbanion-enamine intermediate in pyruvate oxidase highlight unrecognized chemical versatility of thiamin.
Proc Natl Acad Sci U S A: 2012, 109(27);10867-72
[PubMed:22730460]
[WorldCat.org]
[DOI]
(I p)
Dirk Höper, Uwe Völker, Michael Hecker
Comprehensive characterization of the contribution of individual SigB-dependent general stress genes to stress resistance of Bacillus subtilis.
J Bacteriol: 2005, 187(8);2810-26
[PubMed:15805528]
[WorldCat.org]
[DOI]
(P p)
Anja Petersohn, Haike Antelmann, Ulf Gerth, Michael Hecker
Identification and transcriptional analysis of new members of the sigmaB regulon in Bacillus subtilis.
Microbiology (Reading): 1999, 145 ( Pt 4);869-880
[PubMed:10220166]
[WorldCat.org]
[DOI]
(P p)