AdaB

• Description: O6-methylguanine-DNA methyltransferase
  
  

Gene name	adaB
Synonyms
Essential	no
Product	O6-methylguanine-DNA methyltransferase
Function	adaptive response to alkylative DNA damage
Gene expression levels in SubtiExpress: adaB
MW, pI	19 kDa, 6.152
Gene length, protein length	537 bp, 179 aa
Immediate neighbours	adaA, ndhF
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

DNA repair/ recombination, prophage 1

This gene is a member of the following regulons

AdaA regulon

The gene

Basic information

• Locus tag: BSU01820

Database entries

• BsubCyc: BSU01820

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: DNA (containing 6-O-methylguanine) + protein L-cysteine = DNA (without 6-O-methylguanine) + protein S-methyl-L-cysteine (according to Swiss-Prot)

• Protein family: MGMT family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU01820

• Structure:

• UniProt: P19220

• KEGG entry: [3]

• E.C. number: 2.1.1.63

Expression and regulation

• Operon: adaA-adaB PubMed

• Expression browser: adaB PubMed

• Sigma factor: SigA PubMed

• Regulation: positive control by AdaA PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Justin S Lenhart, Jeremy W Schroeder, Brian W Walsh, Lyle A Simmons
DNA repair and genome maintenance in Bacillus subtilis.
Microbiol Mol Biol Rev: 2012, 76(3);530-64
[PubMed:22933559] [WorldCat.org] [DOI] (I p)

Original publications

F Morohoshi, K Hayashi, N Munakata
Bacillus subtilis ada operon encodes two DNA alkyltransferases.
Nucleic Acids Res: 1990, 18(18);5473-80
[PubMed:2120677] [WorldCat.org] [DOI] (P p)