RplB

• Description: ribosomal protein L2, required for assembly of RplP into the 50S subunit of the ribosome
  
  

Gene name	rplB
Synonyms
Essential	yes PubMed
Product	ribosomal protein L2 (BL2)
Function	translation
Gene expression levels in SubtiExpress: rplB
Interactions involving this protein in SubtInteract: RplB
MW, pI	30 kDa, 11.013
Gene length, protein length	831 bp, 277 aa
Immediate neighbours	rplW, rpsS
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

translation, essential genes, universally conserved proteins

This gene is a member of the following regulons

stringent response

The gene

Basic information

• Locus tag: BSU01190

Phenotypes of a mutant

• essential PubMed
• a H142L mutation results in a temperature-sensitive phenotype and is acompanied by growth and sporulation defects, the mutation can be suppressed by increased expression of yaaA PubMed

Database entries

• BsubCyc: BSU01190

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: ribosomal protein L2P family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:
  • RplB-23S rRNA PubMed

• Localization:

Database entries

• BsubCyc: BSU01190

• Structure: 1RL2 (L2 RNA-binding domain, Geobacillus stearothermophilus)

• UniProt: P42919

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: rpsJ-rplC-rplD-rplW-rplB-rpsS-rplV-rpsC-rplP-rpmC-rpsQ-rplN-rplX-rplE-rpsN-rpsH-rplF-rplR-rpsE-rpmD-rplO PubMed

• Expression browser: rplB PubMed

• Sigma factor:
  • SigA two promoters, about 140bp and 200bp upstream of the start codon PubMed

• Regulation:
  • RelA dependent downregulation (Class I) during stringent response PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: