CapB

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  • Description: capsular polyglutamate biosynthesis

Gene name capB
Synonyms ywsC, pgsB
Essential no
Product poly-gamma-glutamate synthetase
Function capsule synthesis
Gene expression levels in SubtiExpress: capB
MW, pI 43 kDa, 5.073
Gene length, protein length 1179 bp, 393 aa
Immediate neighbours capC, rbsR
Sequences Protein DNA DNA_with_flanks
Genetic context
YwsC context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
CapB expression.png















Categories containing this gene/protein

capsule biosynthesis and degradation

This gene is a member of the following regulons

DegU regulon

The gene

Basic information

  • Locus tag: BSU35900

Phenotypes of a mutant

no synthesis of the poly-gamma-glutamate capsule PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: synthesis of poly-gamma-glutamate from L-Glu PubMed
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Modification:
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:
    • The operon is not expressed in the lab strain 168 due to lack of expression of DegQ resulting in inactivity of DegS and DegU PubMed
    • expression of the operon is reduced in motA or motB mutants due to reduced DegU phosphorylation PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Massimiliano Marvasi, Pieter T Visscher, Lilliam Casillas Martinez
Exopolymeric substances (EPS) from Bacillus subtilis: polymers and genes encoding their synthesis.
FEMS Microbiol Lett: 2010, 313(1);1-9
[PubMed:20735481] [WorldCat.org] [DOI] (I p)

Thomas Candela, Agnès Fouet
Poly-gamma-glutamate in bacteria.
Mol Microbiol: 2006, 60(5);1091-8
[PubMed:16689787] [WorldCat.org] [DOI] (P p)


Original publications

Jia Mun Chan, Sarah B Guttenplan, Daniel B Kearns
Defects in the flagellar motor increase synthesis of poly-γ-glutamate in Bacillus subtilis.
J Bacteriol: 2014, 196(4);740-53
[PubMed:24296669] [WorldCat.org] [DOI] (I p)

Thi-Huyen Do, Yuki Suzuki, Naoki Abe, Jun Kaneko, Yoshifumi Itoh, Keitarou Kimura
Mutations suppressing the loss of DegQ function in Bacillus subtilis (natto) poly-γ-glutamate synthesis.
Appl Environ Microbiol: 2011, 77(23);8249-58
[PubMed:21965392] [WorldCat.org] [DOI] (I p)

Tohru Kamei, Daisuke Yamashiro, Terumi Horiuchii, Yutaka Minouchi, Makoto Ashiuchi
Identification and biochemical characterization of membranous short-chain polyglutamate from Bacillus subtilis.
Chem Biodivers: 2010, 7(6);1563-72
[PubMed:20564574] [WorldCat.org] [DOI] (I p)

Chuan-Mei Yeh, Jyh-Perng Wang, Shih-Ching Lo, Wen-Chia Chan, Ming-Yi Lin
Chromosomal integration of a synthetic expression control sequence achieves poly-gamma-glutamate production in a Bacillus subtilis strain.
Biotechnol Prog: 2010, 26(4);1001-7
[PubMed:20564357] [WorldCat.org] [DOI] (I p)

Taku Ohsawa, Kensuke Tsukahara, Mitsuo Ogura
Bacillus subtilis response regulator DegU is a direct activator of pgsB transcription involved in gamma-poly-glutamic acid synthesis.
Biosci Biotechnol Biochem: 2009, 73(9);2096-102
[PubMed:19734658] [WorldCat.org] [DOI] (I p)

Keitarou Kimura, Lam-Son Phan Tran, Thi-Huyen Do, Yoshifumi Itoh
Expression of the pgsB encoding the poly-gamma-DL-glutamate synthetase of Bacillus subtilis (natto).
Biosci Biotechnol Biochem: 2009, 73(5);1149-55
[PubMed:19420703] [WorldCat.org] [DOI] (I p)

Cecilia Osera, Giuseppe Amati, Cinzia Calvio, Alessandro Galizzi
SwrAA activates poly-gamma-glutamate synthesis in addition to swarming in Bacillus subtilis.
Microbiology (Reading): 2009, 155(Pt 7);2282-2287
[PubMed:19389763] [WorldCat.org] [DOI] (P p)

Yuji Urushibata, Shinji Tokuyama, Yasutaka Tahara
Difference in transcription levels of cap genes for gamma-polyglutamic acid production between Bacillus subtilis IFO 16449 and Marburg 168.
J Biosci Bioeng: 2002, 93(2);252-4
[PubMed:16233197] [WorldCat.org] [DOI] (P p)

Yuji Urushibata, Shinji Tokuyama, Yasutaka Tahara
Characterization of the Bacillus subtilis ywsC gene, involved in gamma-polyglutamic acid production.
J Bacteriol: 2002, 184(2);337-43
[PubMed:11751809] [WorldCat.org] [DOI] (P p)