YbfK

• Description: carboxylesterase NP
  
  

Gene name	ybfK
Synonyms	cesB
Essential	no
Product	carboxylesterase NP
Function	degradation of isoprenoid-containing lipids
Gene expression levels in SubtiExpress: ybfK
MW, pI	32 kDa, 5.135
Gene length, protein length	888 bp, 296 aa
Immediate neighbours	ybfJ, pssA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of lipids

The gene

Basic information

• Locus tag: BSU02260

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: A carboxylic ester + H₂O = an alcohol + a carboxylate (according to Swiss-Prot)

• Protein family: AB hydrolase superfamily (according to Swiss-Prot)

• Paralogous protein(s): Nap

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:
• Interactions:

• Localization:
  • cytoplasm (according to Swiss-Prot)

Database entries

• Structure: 4CCY PubMed

• UniProt: O31452

• KEGG entry: [2]

• E.C. number: 3.1.1.1

Expression and regulation

• Operon:

• Expression browser: ybfK PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Henriëtte J Rozeboom, Luis F Godinho, Marco Nardini, Wim J Quax, Bauke W Dijkstra
Crystal structures of two Bacillus carboxylesterases with different enantioselectivities.
Biochim Biophys Acta: 2014, 1844(3);567-75
[PubMed:24418394] [WorldCat.org] [DOI] (P p)

M J Dröge, R Bos, W J Quax
Paralogous gene analysis reveals a highly enantioselective 1,2-O-isopropylideneglycerol caprylate esterase of Bacillus subtilis.
Eur J Biochem: 2001, 268(11);3332-8
[PubMed:11389736] [WorldCat.org] [DOI] (P p)