TrxB

• Description: thioredoxin reductase (NADPH)
  
  

Gene name	trxB
Synonyms	yvcH
Essential	yes PubMed
Product	thioredoxin reductase (NADPH)
Function	keeps thioredoxin in the reduced state
Gene expression levels in SubtiExpress: trxB
MW, pI	34 kDa, 4.99
Gene length, protein length	948 bp, 316 aa
Immediate neighbours	yvcI, cwlO
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

electron transport/ other, resistance against oxidative and electrophile stress, essential genes

This gene is a member of the following regulons

Spx regulon

The gene

Basic information

• Locus tag: BSU34790

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Thioredoxin + NADP⁺ = thioredoxin disulfide + NADPH (according to Swiss-Prot)

• Protein family: class-II pyridine nucleotide-disulfide oxidoreductase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:

• Localization:
  • cytoplasm (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: P80880

• KEGG entry: [2]

• E.C. number: 1.8.1.9

Expression and regulation

• Operon:

• Expression browser: trxB PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:
  • Spx: transcription activation PubMed

• Additional information:
  • A ncRNA is predicted between yvcI and trxB PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Original publications

Mackenzie J Parker, Xuling Zhu, JoAnne Stubbe
Bacillus subtilis class Ib ribonucleotide reductase: high activity and dynamic subunit interactions.
Biochemistry: 2014, 53(4);766-76
[PubMed:24401092] [WorldCat.org] [DOI] (I p)

Irnov Irnov, Cynthia M Sharma, Jörg Vogel, Wade C Winkler
Identification of regulatory RNAs in Bacillus subtilis.
Nucleic Acids Res: 2010, 38(19);6637-51
[PubMed:20525796] [WorldCat.org] [DOI] (I p)

Michiko M Nakano, Ann Lin, Cole S Zuber, Kate J Newberry, Richard G Brennan, Peter Zuber
Promoter recognition by a complex of Spx and the C-terminal domain of the RNA polymerase alpha subunit.
PLoS One: 2010, 5(1);e8664
[PubMed:20084284] [WorldCat.org] [DOI] (I e)

Shunji Nakano, Michiko M Nakano, Ying Zhang, Montira Leelakriangsak, Peter Zuber
A regulatory protein that interferes with activator-stimulated transcription in bacteria.
Proc Natl Acad Sci U S A: 2003, 100(7);4233-8
[PubMed:12642660] [WorldCat.org] [DOI] (P p)