PucH

• Description: allantoinase
  
  

Gene name	pucH
Synonyms	yunH
Essential	no
Product	allantoinase
Function	purine utilization
Gene expression levels in SubtiExpress: pucH
Metabolic function and regulation of this protein in SubtiPathways: pucH
MW, pI	48 kDa, 5.062
Gene length, protein length	1338 bp, 446 aa
Immediate neighbours	yunG, pucR
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of nucleotides

This gene is a member of the following regulons

PucR regulon

The gene

Basic information

• Locus tag: BSU32410

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: (S)-allantoin + H₂O = allantoate (according to Swiss-Prot)

• Protein family: Allantoinase subfamily (according to Swiss-Prot)

• Paralogous protein(s): PyrC

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 3HM7 (from Bacillus halodurans, 44% identity, 65% similarity)

• UniProt: O32137

• KEGG entry: [3]

• E.C. number: 3.5.2.5

Expression and regulation

• Operon: pucH PubMed

• Expression browser: pucH PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced in the presence of purine nucleotides (inducer: allantoin) (PucR) PubMed

• Regulatory mechanism:
  • PucR: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Lars Beier, Per Nygaard, Hanne Jarmer, Hans H Saxild
Transcription analysis of the Bacillus subtilis PucR regulon and identification of a cis-acting sequence required for PucR-regulated expression of genes involved in purine catabolism.
J Bacteriol: 2002, 184(12);3232-41
[PubMed:12029039] [WorldCat.org] [DOI] (P p)

A C Schultz, P Nygaard, H H Saxild
Functional analysis of 14 genes that constitute the purine catabolic pathway in Bacillus subtilis and evidence for a novel regulon controlled by the PucR transcription activator.
J Bacteriol: 2001, 183(11);3293-302
[PubMed:11344136] [WorldCat.org] [DOI] (P p)