RibR

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  • Description: cryptic riboflavin kinase, binds leader (RFN) rib operon mRNA, may have a regulatory role, required for the conversion of S-methyl-cysteine to cysteine

Gene name ribR
Synonyms ytnK
Essential no
Product cryptic riboflavin kinase
Function utilization of S-methyl-cysteine, regulation of rib operon expression
Gene expression levels in SubtiExpress: ribR
Metabolic function and regulation of this protein in SubtiPathways:
ribR
MW, pI 26 kDa, 9.278
Gene length, protein length 690 bp, 230 aa
Immediate neighbours sndA, cmoJ
Sequences Protein DNA DNA_with_flanks
Genetic context
RibR context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
RibR expression.png















Categories containing this gene/protein

poorly characterized/ putative enzymes, sulfur metabolism

This gene is a member of the following regulons

CymR regulon, AscR regulon

The gene

Basic information

  • Locus tag: BSU29300

Phenotypes of a mutant

  • no growth with S-methyl cysteine PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • repressed in the presence of cysteine (CymR) PubMed
    • induced in the presence of S-methyl-cysteine (AscR) PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Che-Man Chan, Antoine Danchin, Philippe Marlière, Agnieszka Sekowska
Paralogous metabolism: S-alkyl-cysteine degradation in Bacillus subtilis.
Environ Microbiol: 2014, 16(1);101-17
[PubMed:23944997] [WorldCat.org] [DOI] (I p)

Yuhei Higashitsuji, Annemarie Angerer, Sabine Berghaus, Birgit Hobl, Matthias Mack
RibR, a possible regulator of the Bacillus subtilis riboflavin biosynthetic operon, in vivo interacts with the 5'-untranslated leader of rib mRNA.
FEMS Microbiol Lett: 2007, 274(1);48-54
[PubMed:17590224] [WorldCat.org] [DOI] (P p)

Pierre Burguière, Juliette Fert, Isabelle Guillouard, Sandrine Auger, Antoine Danchin, Isabelle Martin-Verstraete
Regulation of the Bacillus subtilis ytmI operon, involved in sulfur metabolism.
J Bacteriol: 2005, 187(17);6019-30
[PubMed:16109943] [WorldCat.org] [DOI] (P p)

Irina M Solovieva, Rimma A Kreneva, Lubov Errais Lopes, Daniel A Perumov
The riboflavin kinase encoding gene ribR of Bacillus subtilis is a part of a 10 kb operon, which is negatively regulated by the yrzC gene product.
FEMS Microbiol Lett: 2005, 243(1);51-8
[PubMed:15668000] [WorldCat.org] [DOI] (P p)

I M Solov'eva, R A Kreneva, L L Erraĭs, A S Mironov, D A Perumov
[Study of the mechanism for regulating ribR gene activity in Bacillus subtilis]. [Issledovanie mekhanizma reguliatsii aktivnosti gena ribR u Bacillus subtilis.]
Genetika: 2004, 40(5);716-20
[PubMed:15272571] [WorldCat.org] (P p)