ProI

• Description: pyrroline-5-carboxylate reductase
  
  

Gene name	proI
Synonyms	yqjO
Essential	no
Product	pyrroline-5-carboxylate reductase
Function	biosynthesis of proline
Gene expression levels in SubtiExpress: proI
Metabolic function and regulation of this protein in SubtiPathways: proI
MW, pI	30 kDa, 6.702
Gene length, protein length	834 bp, 278 aa
Immediate neighbours	yqjP, yqjN
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis/ acquisition of amino acids

This gene is a member of the following regulons

T-box

The gene

Basic information

• Locus tag: BSU23800

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: L-proline + NAD(P)⁺ = 1-pyrroline-5-carboxylate + NAD(P)H (according to Swiss-Prot)

• Protein family: pyrroline-5-carboxylate reductase family (according to Swiss-Prot)

• Paralogous protein(s): ProH

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: P54552

• KEGG entry: [3]

• E.C. number: 1.5.1.2

Expression and regulation

• Operon: proI PubMed

• Expression browser: proI PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced by proline limitation (T-box) PubMed

• Regulatory mechanism:
  • T-box: RNA switch, transcriptional antitermination PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Additional publications: PubMed

Jeanette Brill, Tamara Hoffmann, Harald Putzer, Erhard Bremer
T-box-mediated control of the anabolic proline biosynthetic genes of Bacillus subtilis.
Microbiology (Reading): 2011, 157(Pt 4);977-987
[PubMed:21233158] [WorldCat.org] [DOI] (I p)

Ana Gutiérrez-Preciado, Tina M Henkin, Frank J Grundy, Charles Yanofsky, Enrique Merino
Biochemical features and functional implications of the RNA-based T-box regulatory mechanism.
Microbiol Mol Biol Rev: 2009, 73(1);36-61
[PubMed:19258532] [WorldCat.org] [DOI] (I p)

B R Belitsky, J Brill, E Bremer, A L Sonenshein
Multiple genes for the last step of proline biosynthesis in Bacillus subtilis.
J Bacteriol: 2001, 183(14);4389-92
[PubMed:11418582] [WorldCat.org] [DOI] (P p)