MleN

• Description: malate-H+/Na+-lactate antiporter
  
  

Gene name	mleN
Synonyms	yqkI
Essential	no
Product	malate-H+/Na+-lactate antiporter
Function	malate uptake
Gene expression levels in SubtiExpress: mleN
Metabolic function and regulation of this protein in SubtiPathways: mleN
MW, pI	50 kDa, 7.249
Gene length, protein length	1404 bp, 468 aa
Immediate neighbours	mleA, ansB
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

transporters/ other, utilization of specific carbon sources, membrane proteins

This gene is a member of the following regulons

AnsR regulon, CcpA regulon

The gene

Basic information

• Locus tag: BSU23560

Phenotypes of a mutant

abrupt arrest in the mid-logarithmic phase of growth on malate when low concentrations of protonophore were present PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: nhaC Na(+)/H(+) antiporter family (according to Swiss-Prot)

• Paralogous protein(s): NhaC

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • membrane associated PubMed

Database entries

• Structure:

• UniProt: P54571

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:
  • ansA-ansB-mleN-mleA PubMed
  • mleN-mleA PubMed

• Expression browser: mleN PubMed

• Sigma factor:
  • ansA: SigA PubMed
  • mleN: SigA PubMed

• Regulation:
  • ansA: expressed in the presence of asparagine (AnsR) PubMed
  • mleN: repressed by glucose (CcpA) PubMed

• Regulatory mechanism:
  • AnsR: transcription repression PubMed
  • CcpA: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:GP1460 (mleN::spc), available in Stülke lab

• Expression vector:

• lacZ fusion: pGP388 (in pAC7), available in Stülke lab

• GFP fusion:

• two-hybrid system:

• Antibody: