Ogt

• Description: O6-methylguanine DNA alkyltransferase
  
  

Gene name	ogt
Synonyms	dat
Essential	no
Product	O6-methylguanine DNA alkyltransferase
Function	DNA repair
Gene expression levels in SubtiExpress: ogt
Function and regulation of this protein in SubtiPathways: ogt
MW, pI	18 kDa, 7.092
Gene length, protein length	495 bp, 165 aa
Immediate neighbours	kinE, mtnA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

DNA repair/ recombination

The gene

Basic information

• Locus tag: BSU13540

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: DNA (containing 6-O-methylguanine) + protein L-cysteine = DNA (without 6-O-methylguanine) + protein S-methyl-L-cysteine (according to Swiss-Prot)

• Protein family: MGMT family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 1SFE (from E. coli, 36% identity, 51% similarity) PubMed

• UniProt: P11742

• KEGG entry: [3]

• E.C. number: 2.1.1.63

Expression and regulation

• Operon: kinE-ogt PubMed

• Expression browser: ogt PubMed

• Sigma factor: SigA PubMed

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

J A Rafferty, J E Wibley, P Speers, I Hickson, G P Margison, P C Moody, K T Douglas
The potential role of glycine-160 of human O6-alkylguanine-DNA alkyltransferase in reaction with O6-benzylguanine as determined by site-directed mutagenesis and molecular modelling comparisons.
Biochim Biophys Acta: 1997, 1342(1);90-102
[PubMed:9366274] [WorldCat.org] [DOI] (P p)

F Morohoshi, N Munakata
Isolation of a Bacillus subtilis mutant defective in constitutive O6-alkylguanine-DNA alkyltransferase.
Mutat Res: 1990, 235(1);15-23
[PubMed:2105461] [WorldCat.org] [DOI] (P p)

F Morohoshi, K Hayashi, N Munakata
Bacillus subtilis gene coding for constitutive O6-methylguanine-DNA alkyltransferase.
Nucleic Acids Res: 1989, 17(16);6531-43
[PubMed:2506524] [WorldCat.org] [DOI] (P p)