CotS

• Description: outer spore coat protein
  
  

Gene name	cotS
Synonyms
Essential	no
Product	spore coat protein
Function	resistance of the spore
Gene expression levels in SubtiExpress: cotS
MW, pI	40 kDa, 6.641
Gene length, protein length	1053 bp, 351 aa
Immediate neighbours	ytxO, cotSA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

sporulation proteins

This gene is a member of the following regulons

SigK regulon, GerE regulon

The gene

Basic information

• Locus tag: BSU30900

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: cotS family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:

• Localization:
  • outer spore coat, localization depends on CotE PubMed

Database entries

• Structure:

• UniProt: P46914

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: cotSA-cotS-ytxO PubMed

• Expression browser: cotS PubMed

• Sigma factor: SigK PubMed

• Regulation:
  • expressed late during sporulation in the mother cell (SigK, GerE) PubMed

• Regulatory mechanism:
  • GerE: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Peter T McKenney, Adam Driks, Patrick Eichenberger
The Bacillus subtilis endospore: assembly and functions of the multilayered coat.
Nat Rev Microbiol: 2013, 11(1);33-44
[PubMed:23202530] [WorldCat.org] [DOI] (I p)

Original publications

Peter T McKenney, Patrick Eichenberger
Dynamics of spore coat morphogenesis in Bacillus subtilis.
Mol Microbiol: 2012, 83(2);245-60
[PubMed:22171814] [WorldCat.org] [DOI] (I p)

Leif Steil, Mónica Serrano, Adriano O Henriques, Uwe Völker
Genome-wide analysis of temporally regulated and compartment-specific gene expression in sporulating cells of Bacillus subtilis.
Microbiology (Reading): 2005, 151(Pt 2);399-420
[PubMed:15699190] [WorldCat.org] [DOI] (P p)

Patrick Eichenberger, Masaya Fujita, Shane T Jensen, Erin M Conlon, David Z Rudner, Stephanie T Wang, Caitlin Ferguson, Koki Haga, Tsutomu Sato, Jun S Liu, Richard Losick
The program of gene transcription for a single differentiating cell type during sporulation in Bacillus subtilis.
PLoS Biol: 2004, 2(10);e328
[PubMed:15383836] [WorldCat.org] [DOI] (I p)

Ritsuko Kuwana, Hiromi Ikejiri, Satoko Yamamura, Hiromu Takamatsu, Kazuhito Watabe
Functional relationship between SpoVIF and GerE in gene regulation during sporulation of Bacillus subtilis.
Microbiology (Reading): 2004, 150(Pt 1);163-170
[PubMed:14702409] [WorldCat.org] [DOI] (P p)

S Little, A Driks
Functional analysis of the Bacillus subtilis morphogenetic spore coat protein CotE.
Mol Microbiol: 2001, 42(4);1107-20
[PubMed:11737650] [WorldCat.org] [DOI] (P p)

H Takamatsu, T Kodama, K Watabe
Assembly of the CotSA coat protein into spores requires CotS in Bacillus subtilis.
FEMS Microbiol Lett: 1999, 174(1);201-6
[PubMed:10234840] [WorldCat.org] [DOI] (P p)

H Takamatsu, Y Chikahiro, T Kodama, H Koide, S Kozuka, K Tochikubo, K Watabe
A spore coat protein, CotS, of Bacillus subtilis is synthesized under the regulation of sigmaK and GerE during development and is located in the inner coat layer of spores.
J Bacteriol: 1998, 180(11);2968-74
[PubMed:9603889] [WorldCat.org] [DOI] (P p)

Akiko Abe, Hidekatsu Koide, Takeyuki Kohno, Kazuhito Watabe
A Bacillus subtilis spore coat polypeptide gene, cotS.
Microbiology (Reading): 1995, 141 ( Pt 6);1433-1442
[PubMed:7545510] [WorldCat.org] [DOI] (P p)