RsbP

• Description: protein serine phosphatase, energy PP2C, dephosphorylates RsbV
  
  

Gene name	rsbP
Synonyms	yvfP
Essential	no
Product	protein serine phosphatase, energy PP2C
Function	control of SigB activity
Gene expression levels in SubtiExpress: rsbP
Interactions involving this protein in SubtInteract: RsbP
Metabolic function and regulation of this protein in SubtiPathways: Stress
MW, pI	45 kDa, 4.827
Gene length, protein length	1209 bp, 403 aa
Immediate neighbours	rsbQ, ganB
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

protein modification, sigma factors and their control

The gene

Basic information

• Locus tag: BSU34110

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: dephosphorylation of RsbV in response to red light, this results in SigB activation PubMed

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains: N-terminal PAS domain, central coiled-coil domain, C-terminal PP2C phosphatase domain

• Modification:

• Cofactor(s):

• Effectors of protein activity:
  • activity is stimulated upon interaction of the RsbP oligomer with RsbQ PubMed

• Interactions:
  • RsbP-RsbV PubMed
  • RsbQ-RsbP PubMed

• Localization:

Database entries

• Structure:

• UniProt: O07014

• KEGG entry: [2]

• E.C. number: 3.1.3.3

Expression and regulation

• Operon:

• Expression browser: rsbP PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

• Chet Price, Davis, USA homepage

References

Reviews

Jon Marles-Wright, Richard J Lewis
The stressosome: molecular architecture of a signalling hub.
Biochem Soc Trans: 2010, 38(4);928-33
[PubMed:20658979] [WorldCat.org] [DOI] (I p)

Original publications

Additional publications: PubMed

Locke JC, Young JW, Fontes M, Hernández Jiménez MJ, Elowitz MB  
Stochastic pulse regulation in bacterial stress response. 
Science. 2011 334:366-369. 
PubMed:21979936

Marcela Avila-Pérez, Jeroen B van der Steen, Remco Kort, Klaas J Hellingwerf
Red light activates the sigmaB-mediated general stress response of Bacillus subtilis via the energy branch of the upstream signaling cascade.
J Bacteriol: 2010, 192(3);755-62
[PubMed:19948797] [WorldCat.org] [DOI] (I p)

Masatomo Makino, Shinpei Kondo, Tomonori Kaneko, Seiki Baba, Kunio Hirata, Takashi Kumasaka
Expression, crystallization and preliminary crystallographic analysis of the PAS domain of RsbP, a stress-response phosphatase from Bacillus subtilis.
Acta Crystallogr Sect F Struct Biol Cryst Commun: 2009, 65(Pt 6);559-61
[PubMed:19478430] [WorldCat.org] [DOI] (I p)

Margaret S Brody, Valley Stewart, Chester W Price
Bypass suppression analysis maps the signalling pathway within a multidomain protein: the RsbP energy stress phosphatase 2C from Bacillus subtilis.
Mol Microbiol: 2009, 72(5);1221-34
[PubMed:19432806] [WorldCat.org] [DOI] (I p)

Shuyu Zhang, W G Haldenwang
Contributions of ATP, GTP, and redox state to nutritional stress activation of the Bacillus subtilis sigmaB transcription factor.
J Bacteriol: 2005, 187(22);7554-60
[PubMed:16267279] [WorldCat.org] [DOI] (P p)

Tomonori Kaneko, Nobuo Tanaka, Takashi Kumasaka
Crystal structures of RsbQ, a stress-response regulator in Bacillus subtilis.
Protein Sci: 2005, 14(2);558-65
[PubMed:15632289] [WorldCat.org] [DOI] (P p)

Gudrun Holtmann, Matthias Brigulla, Leif Steil, Alexandra Schütz, Karsta Barnekow, Uwe Völker, Erhard Bremer
RsbV-independent induction of the SigB-dependent general stress regulon of Bacillus subtilis during growth at high temperature.
J Bacteriol: 2004, 186(18);6150-8
[PubMed:15342585] [WorldCat.org] [DOI] (P p)

Matthias Brigulla, Tamara Hoffmann, Andrea Krisp, Andrea Völker, Erhard Bremer, Uwe Völker
Chill induction of the SigB-dependent general stress response in Bacillus subtilis and its contribution to low-temperature adaptation.
J Bacteriol: 2003, 185(15);4305-14
[PubMed:12867438] [WorldCat.org] [DOI] (P p)

M S Brody, K Vijay, C W Price
Catalytic function of an alpha/beta hydrolase is required for energy stress activation of the sigma(B) transcription factor in Bacillus subtilis.
J Bacteriol: 2001, 183(21);6422-8
[PubMed:11591687] [WorldCat.org] [DOI] (P p)

K Vijay, M S Brody, E Fredlund, C W Price
A PP2C phosphatase containing a PAS domain is required to convey signals of energy stress to the sigmaB transcription factor of Bacillus subtilis.
Mol Microbiol: 2000, 35(1);180-8
[PubMed:10632888] [WorldCat.org] [DOI] (P p)