Spo0E

• Description: Spo0A-P phosphatase, control of the phosphorelay
  
  

Gene name	spo0E
Synonyms
Essential	no
Product	Spo0A-P phosphatase
Function	initiation of sporulation
Gene expression levels in SubtiExpress: spo0E
Interactions involving this protein in SubtInteract: Spo0E
Function and regulation of this protein in SubtiPathways: Phosphorelay
MW, pI	9 kDa, 6.483
Gene length, protein length	255 bp, 85 aa
Immediate neighbours	ykvA, eag
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

protein modification, phosphorelay

This gene is a member of the following regulons

SigB regulon, AbrB regulon, Rok regulon

The gene

Basic information

• Locus tag: BSU13640

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: spo0E family (according to Swiss-Prot)

• Paralogous protein(s): YnzD

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • Spo0F-Spo0E PubMed
  • Spo0A-Spo0E PubMed
  • FtsH-Spo0E PubMed

• Localization:

Database entries

• Structure:

• UniProt: P05043

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: spo0E PubMed

• Expression browser: spo0E PubMed

• Sigma factor:
  • SigA PubMed, SigB PubMed

• Regulation:
  • repressed during logrithmic growth (AbrB) PubMed
  • induced under stress conditions (SigB) PubMed

• Regulatory mechanism:
  • AbrB: transcription repression PubMed
  • Rok: transcription repression of the SigB-dependent promoter PubMed

• Additional information:
  • Spo0E is degraded by FtsH
  • stress-induced expression of Spo0E by SigB results in prevention of sporulation during stress (due to dephosphorylation of Spo0A) PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Tony Wilkinson, York University, U.K. homepage

References

Reviews

Daniel Schultz, Peter G Wolynes, Eshel Ben Jacob, José N Onuchic
Deciding fate in adverse times: sporulation and competence in Bacillus subtilis.
Proc Natl Acad Sci U S A: 2009, 106(50);21027-34
[PubMed:19995980] [WorldCat.org] [DOI] (I p)

Original Publications

Additional publications: PubMed