YerD

• Description: general stress protein, similar to glutamate synthase (ferredoxin), required for protection against paraquat stress
  
  

Gene name	yerD
Synonyms
Essential	no
Product	unknown
Function	protection against paraquat stress
Gene expression levels in SubtiExpress: yerD
MW, pI	58 kDa, 7.349
Gene length, protein length	1575 bp, 525 aa
Immediate neighbours	yerC, pcrB
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

general stress proteins (controlled by SigB), resistance against oxidative and electrophile stress, poorly characterized/ putative enzymes, membrane proteins

This gene is a member of the following regulons

SigB regulon

The gene

Basic information

• Locus tag: BSU06590

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s): GltA

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • cell membrane (according to UniProt)

Database entries

• Structure:

• UniProt: O34849

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: yerD PubMed

• Expression browser: yerD PubMed

• Sigma factor: SigB PubMed

• Regulation:
  • induced by stress (SigB) PubMed
  • strongly induced in response to glucose starvation in M9 medium PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant: GP1184 (tet) available in Jörg Stülke's lab

• Expression vector: pBP32 (expression vector for E. coli BL21; N-terminal Strep-tag-YerD fusion protein, based on pGP172), available in Fabian Commichau's lab

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• FLAG-tag construct: GP1187 (spc, based on pGP1331), available in Jörg Stülke's lab

• Antibody:

Labs working on this gene/protein

Fabian Commichau University of Göttingen, Germany Homepage