RefZ

• Description: regulator of FtsZ (TetR family), facilitates switch from medial to polar FtsZ ring placement during sporulation
  
  

Gene name	refZ
Synonyms	yttP
Essential	no
Product	regulator of FtsZ
Function	relocalization of the FtsZ ring
Interactions involving this protein in SubtInteract: RefZ
MW, pI	24 kDa, 6.706
Gene length, protein length	621 bp, 207 aa
Immediate neighbours	hisJ, ytsP
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

cell division, sporulation/ other

This gene is a member of the following regulons

CcpA regulon, PhoP regulon, Spo0A regulon

The gene

Basic information

• Locus tag: BSU29630

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: facilitates switch from medial to polar FtsZ ring placement during sporulation PubMed

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:
  • TetR-like DNA-binding helix-turn-helix domain at the N-terminus PubMed

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • RefZ-FtsZ PubMed

• Localization:
  • polar foci PubMed

Database entries

• Structure:

• UniProt: O34970

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: refZ PubMed

• Expression browser: refZ PubMed

• Sigma factor:

• Regulation:
  • repressed by glucose (3.1-fold) (CcpA) PubMed
  • induced early during sporulation (Spo0A) PubMed
  • expressed under conditions of phosphate limitation (PhoP) PubMed

• Regulatory mechanism:
  • CcpA: transcription repression PubMed
  • Spo0A: transcription activation PubMed
  • PhoP: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Leslie Cuthbertson, Justin R Nodwell
The TetR family of regulators.
Microbiol Mol Biol Rev: 2013, 77(3);440-75
[PubMed:24006471] [WorldCat.org] [DOI] (I p)

Original publications

Jennifer K Wagner-Herman, Remi Bernard, Roisin Dunne, Alexandre W Bisson-Filho, Krithika Kumar, Trang Nguyen, Lawrence Mulcahy, John Koullias, Frederico J Gueiros-Filho, David Z Rudner
RefZ facilitates the switch from medial to polar division during spore formation in Bacillus subtilis.
J Bacteriol: 2012, 194(17);4608-18
[PubMed:22730127] [WorldCat.org] [DOI] (I p)

Pierre Nicolas, Ulrike Mäder, Etienne Dervyn, Tatiana Rochat, Aurélie Leduc, Nathalie Pigeonneau, Elena Bidnenko, Elodie Marchadier, Mark Hoebeke, Stéphane Aymerich, Dörte Becher, Paola Bisicchia, Eric Botella, Olivier Delumeau, Geoff Doherty, Emma L Denham, Mark J Fogg, Vincent Fromion, Anne Goelzer, Annette Hansen, Elisabeth Härtig, Colin R Harwood, Georg Homuth, Hanne Jarmer, Matthieu Jules, Edda Klipp, Ludovic Le Chat, François Lecointe, Peter Lewis, Wolfram Liebermeister, Anika March, Ruben A T Mars, Priyanka Nannapaneni, David Noone, Susanne Pohl, Bernd Rinn, Frank Rügheimer, Praveen K Sappa, Franck Samson, Marc Schaffer, Benno Schwikowski, Leif Steil, Jörg Stülke, Thomas Wiegert, Kevin M Devine, Anthony J Wilkinson, Jan Maarten van Dijl, Michael Hecker, Uwe Völker, Philippe Bessières, Philippe Noirot
Condition-dependent transcriptome reveals high-level regulatory architecture in Bacillus subtilis.
Science: 2012, 335(6072);1103-6
[PubMed:22383849] [WorldCat.org] [DOI] (I p)

Zoltán Prágai, Nicholas E E Allenby, Nicola O'Connor, Sarah Dubrac, Georges Rapoport, Tarek Msadek, Colin R Harwood
Transcriptional regulation of the phoPR operon in Bacillus subtilis.
J Bacteriol: 2004, 186(4);1182-90
[PubMed:14762014] [WorldCat.org] [DOI] (P p)

Virginie Molle, Masaya Fujita, Shane T Jensen, Patrick Eichenberger, José E González-Pastor, Jun S Liu, Richard Losick
The Spo0A regulon of Bacillus subtilis.
Mol Microbiol: 2003, 50(5);1683-701
[PubMed:14651647] [WorldCat.org] [DOI] (P p)

Hans-Matti Blencke, Georg Homuth, Holger Ludwig, Ulrike Mäder, Michael Hecker, Jörg Stülke
Transcriptional profiling of gene expression in response to glucose in Bacillus subtilis: regulation of the central metabolic pathways.
Metab Eng: 2003, 5(2);133-49
[PubMed:12850135] [WorldCat.org] [DOI] (P p)