QueC

• Description: synthesis of the modified ribonucleotide queuosine
  
  

Gene name	queC
Synonyms	ykvJ
Essential	no
Product	unknown
Function	tRNA modification
Gene expression levels in SubtiExpress: queC
MW, pI	24 kDa, 5.147
Gene length, protein length	657 bp, 219 aa
Immediate neighbours	ykvI, queD
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

translation

This gene is a member of the following regulons

preQ1 riboswitch

The gene

Basic information

• Locus tag: BSU13720

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: queC family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 3BL5

• UniProt: O31675

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: queC-queD-queE-queF PubMed

• Expression browser: queC PubMed

• Sigma factor:

• Regulation:
  • repressed in the presence of queuosine (preQ1 riboswitch) PubMed

• Regulatory mechanism:
  • preQ1 riboswitch: transcriptional antitermination in the absence of queuosine PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Krishna C Suddala, Arlie J Rinaldi, Jun Feng, Anthony M Mustoe, Catherine D Eichhorn, Joseph A Liberman, Joseph E Wedekind, Hashim M Al-Hashimi, Charles L Brooks, Nils G Walter
Single transcriptional and translational preQ1 riboswitches adopt similar pre-folded ensembles that follow distinct folding pathways into the same ligand-bound structure.
Nucleic Acids Res: 2013, 41(22);10462-75
[PubMed:24003028] [WorldCat.org] [DOI] (I p)

Qi Zhang, Mijeong Kang, Robert D Peterson, Juli Feigon
Comparison of solution and crystal structures of preQ1 riboswitch reveals calcium-induced changes in conformation and dynamics.
J Am Chem Soc: 2011, 133(14);5190-3
[PubMed:21410253] [WorldCat.org] [DOI] (I p)

Jun Feng, Nils G Walter, Charles L Brooks
Cooperative and directional folding of the preQ1 riboswitch aptamer domain.
J Am Chem Soc: 2011, 133(12);4196-9
[PubMed:21375305] [WorldCat.org] [DOI] (I p)

Reid M McCarty, Arpád Somogyi, Guangxin Lin, Neil E Jacobsen, Vahe Bandarian
The deazapurine biosynthetic pathway revealed: in vitro enzymatic synthesis of PreQ(0) from guanosine 5'-triphosphate in four steps.
Biochemistry: 2009, 48(18);3847-52
[PubMed:19354300] [WorldCat.org] [DOI] (I p)

Mijeong Kang, Robert Peterson, Juli Feigon
Structural Insights into riboswitch control of the biosynthesis of queuosine, a modified nucleotide found in the anticodon of tRNA.
Mol Cell: 2009, 33(6);784-90
[PubMed:19285444] [WorldCat.org] [DOI] (I p)

Nenad Cicmil, Raven H Huang
Crystal structure of QueC from Bacillus subtilis: an enzyme involved in preQ1 biosynthesis.
Proteins: 2008, 72(3);1084-8
[PubMed:18491386] [WorldCat.org] [DOI] (I p)

Adam Roth, Wade C Winkler, Elizabeth E Regulski, Bobby W K Lee, Jinsoo Lim, Inbal Jona, Jeffrey E Barrick, Ankita Ritwik, Jane N Kim, Rüdiger Welz, Dirk Iwata-Reuyl, Ronald R Breaker
A riboswitch selective for the queuosine precursor preQ1 contains an unusually small aptamer domain.
Nat Struct Mol Biol: 2007, 14(4);308-17
[PubMed:17384645] [WorldCat.org] [DOI] (P p)

John S Reader, David Metzgar, Paul Schimmel, Valérie de Crécy-Lagard
Identification of four genes necessary for biosynthesis of the modified nucleoside queuosine.
J Biol Chem: 2004, 279(8);6280-5
[PubMed:14660578] [WorldCat.org] [DOI] (P p)