DnaD

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  • Description: initiation of chromosome replication, part of the replisome

Gene name dnaD
Synonyms
Essential yes PubMed
Product initiation of chromosome replication
Function DNA replication
Gene expression levels in SubtiExpress: dnaD
Interactions involving this protein in SubtInteract: DnaD
MW, pI 27 kDa, 7.174
Gene length, protein length 696 bp, 232 aa
Immediate neighbours nth, asnS
Sequences Protein DNA DNA_with_flanks
Genetic context
DnaD context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
DnaD expression.png














Categories containing this gene/protein

DNA replication, essential genes

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU22350

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • DnaD-mediated untwisting of the DNA stimulates Nth endonuclease activity at AP sites PubMed
    • required for the assembly of the replicative helicase DnaC at the origin of replication PubMed
    • interaction with DnaA inhibits the ability of DnaA to bind cooperatively to DNA PubMed
  • Protein family: DNA polymerase type-Y family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 2C79 (N-terminal domain)
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • constitutively expressed PubMed
    • there are about 3,000 to 5,000 molecules of DnaD per cell PubMed
    • expression is transiently increased after treatment with H2O2 PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Geoffrey S Briggs, Wiep Klaas Smits, Panos Soultanas
Chromosomal replication initiation machinery of low-G+C-content Firmicutes.
J Bacteriol: 2012, 194(19);5162-70
[PubMed:22797751] [WorldCat.org] [DOI] (I p)

Original publications