GlpD

• Description: glycerol-3-phosphate dehydrogenase (menaquinone 7)
  
  

Gene name	glpD
Synonyms
Essential	no
Product	glycerol-3-phosphate dehydrogenase (menaquinone 7)
Function	glycerol utilization
Gene expression levels in SubtiExpress: glpD
Metabolic function and regulation of this protein in SubtiPathways: Sugar catabolism
MW, pI	62 kDa, 8.002
Gene length, protein length	1665 bp, 555 aa
Immediate neighbours	glpK, pgcA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of specific carbon sources, utilization of lipids

This gene is a member of the following regulons

AbrB regulon, CcpA regulon, GlpP regulon

The gene

Basic information

• Locus tag: BSU09300

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: sn-glycerol 3-phosphate + a quinone = glycerone phosphate + a quinol (according to Swiss-Prot)

• Protein family: FAD-dependent glycerol-3-phosphate dehydrogenase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s): FAD

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure: 3DA1 (from Bacillus halodurans, 57% identity, 72% similarity)

• UniProt: P18158

• KEGG entry: [3]

• E.C. number: 1.1.5.3

Expression and regulation

• Operon: glpD PubMed

• Expression browser: glpD PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed by glucose (CcpA)
  • induced by glycerol (GlpP) PubMed

• Regulatory mechanism:
  • CcpA: transcription repression
  • GlpP: transcriptional antitermination at a RNA switch PubMed
  • AbrB: transcription activation PubMed

• Additional information:

Biological materials

• Mutant: QB5347 (cat), available in the Stülke lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: