FadF

• Description: similar to iron-sulphur-binding reductase
  
  

Gene name	fadF
Synonyms	ywjF
Essential	no
Product	unknown
Function	fatty acid degradation
Gene expression levels in SubtiExpress: fadF
Metabolic function and regulation of this protein in SubtiPathways: Fatty acid degradation
MW, pI	79 kDa, 6.526
Gene length, protein length	2115 bp, 705 aa
Immediate neighbours	acdA, ywjE
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of lipids, membrane proteins

This gene is a member of the following regulons

FadR regulon

The gene

Basic information

• Locus tag: BSU37180

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s): contains an iron-sulfur cluster

• Effectors of protein activity:

• Interactions:

• Localization:
  • cell membrane (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: P45866

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: fadF-acdA-rpoE PubMed

• Expression browser: fadF PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed in the absence of long-chain fatty acids (FadR) PubMed
  • repressed by glucose (5-fold) PubMed
  • strongly induced in response to glucose starvation in M9 medium PubMed

• Regulatory mechanism:
  • FadR: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Imke G de Jong, Jan-Willem Veening, Oscar P Kuipers
Single cell analysis of gene expression patterns during carbon starvation in Bacillus subtilis reveals large phenotypic variation.
Environ Microbiol: 2012, 14(12);3110-21
[PubMed:23033921] [WorldCat.org] [DOI] (I p)

Hiroshi Matsuoka, Kazutake Hirooka, Yasutaro Fujita
Organization and function of the YsiA regulon of Bacillus subtilis involved in fatty acid degradation.
J Biol Chem: 2007, 282(8);5180-94
[PubMed:17189250] [WorldCat.org] [DOI] (P p)

Hans-Matti Blencke, Georg Homuth, Holger Ludwig, Ulrike Mäder, Michael Hecker, Jörg Stülke
Transcriptional profiling of gene expression in response to glucose in Bacillus subtilis: regulation of the central metabolic pathways.
Metab Eng: 2003, 5(2);133-49
[PubMed:12850135] [WorldCat.org] [DOI] (P p)