FruK

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  • Description: fructose-1-phosphate kinase

Gene name fruK
Synonyms fruB
Essential no
Product fructose-1-phosphate kinase
Function fructose utilization
Gene expression levels in SubtiExpress: fruK
Metabolic function and regulation of this protein in SubtiPathways:
Sugar catabolism
MW, pI 32 kDa, 4.885
Gene length, protein length 909 bp, 303 aa
Immediate neighbours fruR, fruA
Sequences Protein DNA DNA_with_flanks
Genetic context
FruK context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
FruK expression.png



















Categories containing this gene/protein

utilization of specific carbon sources

This gene is a member of the following regulons

FruR regulon

The gene

Basic information

  • Locus tag: BSU14390

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + D-fructose 1-phosphate = ADP + D-fructose 1,6-bisphosphate (according to Swiss-Prot)
  • Protein family: carbohydrate kinase pfkB family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • KEGG entry: [3]

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation:
    • induced in the presence of fructose (FruR) (according to DBTBS)
  • Regulatory mechanism:
    • FruR: transcription repression (according to DBTBS)
    • strongly induced in response to glucose starvation in M9 medium PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Imke G de Jong, Jan-Willem Veening, Oscar P Kuipers
Single cell analysis of gene expression patterns during carbon starvation in Bacillus subtilis reveals large phenotypic variation.
Environ Microbiol: 2012, 14(12);3110-21
[PubMed:23033921] [WorldCat.org] [DOI] (I p)

Jonathan Reizer, Steffi Bachem, Aiala Reizer, Maryvonne Arnaud, Milton H Saier, Jörg Stülke
Novel phosphotransferase system genes revealed by genome analysis - the complete complement of PTS proteins encoded within the genome of Bacillus subtilis.
Microbiology (Reading): 1999, 145 ( Pt 12);3419-3429
[PubMed:10627040] [WorldCat.org] [DOI] (P p)