GlpX

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  • Description: class II fructose-1,6-bisphosphatase, gluconeogenesis

Gene name glpX
Synonyms ywjI
Essential no
Product class II fructose-1,6-bisphosphatase
Function gluconeogenesis
Gene expression levels in SubtiExpress: glpX
Metabolic function and regulation of this protein in SubtiPathways:
Central C-metabolism, Sugar catabolism
MW, pI 33 kDa, 5.067
Gene length, protein length 963 bp, 321 aa
Immediate neighbours rho, murAB
Sequences Protein DNA DNA_with_flanks
Genetic context
YwjI context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
GlpX expression.png




























Categories containing this gene/protein

carbon core metabolism

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU37090

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: D-fructose 1,6-bisphosphate ==> D-fructose 6-phosphate + Pi
  • Protein family: FBPase class 2 family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information: K(M) for FBP: 20 µM PubMed
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 3D1R (from E. coli, 49% identity, 68% similarity)PubMed
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation: constitutive PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Matthieu Jules, Ludovic Le Chat, Stéphane Aymerich, Dominique Le Coq
The Bacillus subtilis ywjI (glpX) gene encodes a class II fructose-1,6-bisphosphatase, functionally equivalent to the class III Fbp enzyme.
J Bacteriol: 2009, 191(9);3168-71
[PubMed:19270101] [WorldCat.org] [DOI] (I p)

Greg Brown, Alexander Singer, Vladimir V Lunin, Michael Proudfoot, Tatiana Skarina, Robert Flick, Samvel Kochinyan, Ruslan Sanishvili, Andrzej Joachimiak, Aled M Edwards, Alexei Savchenko, Alexander F Yakunin
Structural and biochemical characterization of the type II fructose-1,6-bisphosphatase GlpX from Escherichia coli.
J Biol Chem: 2009, 284(6);3784-92
[PubMed:19073594] [WorldCat.org] [DOI] (P p)