TagB

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  • Description: putative CDP-glycerol:glycerol phosphate glycerophosphotransferase

Gene name tagB
Synonyms
Essential yes PubMed
Product putative CDP-glycerol:glycerol
phosphate glycerophosphotransferase
Function biosynthesis of teichoic acid
Gene expression levels in SubtiExpress: tagB
MW, pI 44 kDa, 8.925
Gene length, protein length 1143 bp, 381 aa
Immediate neighbours tagA, tagC
Sequences Protein DNA DNA_with_flanks
Genetic context
TagB context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
TagB expression.png

























Categories containing this gene/protein

cell wall synthesis, biosynthesis of cell wall components, essential genes, membrane proteins

This gene is a member of the following regulons

PhoP regulon, WalR regulon

The gene

Basic information

  • Locus tag: BSU35760

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: adds a single glycerol phosphate residue to the non-reducing end of the undecaprenol-phosphate-linked N-acetylmannosamine-β(1,4)-N-acetylglucosamine-1-phosphate PubMed
  • Protein family: CDP-glycerol glycerophosphotransferase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • repressed when phosphate is available (PhoP) PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Alex Formstone, Rut Carballido-López, Philippe Noirot, Jeffery Errington, Dirk-Jan Scheffers
Localization and interactions of teichoic acid synthetic enzymes in Bacillus subtilis.
J Bacteriol: 2008, 190(5);1812-21
[PubMed:18156271] [WorldCat.org] [DOI] (I p)

Amit P Bhavsar, Michael A D'Elia, Tiffany D Sahakian, Eric D Brown
The Amino terminus of Bacillus subtilis TagB possesses separable localization and functional properties.
J Bacteriol: 2007, 189(19);6816-23
[PubMed:17660278] [WorldCat.org] [DOI] (P p)

Amit P Bhavsar, Ray Truant, Eric D Brown
The TagB protein in Bacillus subtilis 168 is an intracellular peripheral membrane protein that can incorporate glycerol phosphate onto a membrane-bound acceptor in vitro.
J Biol Chem: 2005, 280(44);36691-700
[PubMed:16150696] [WorldCat.org] [DOI] (P p)

Amit P Bhavsar, Laura K Erdman, Jeffrey W Schertzer, Eric D Brown
Teichoic acid is an essential polymer in Bacillus subtilis that is functionally distinct from teichuronic acid.
J Bacteriol: 2004, 186(23);7865-73
[PubMed:15547257] [WorldCat.org] [DOI] (P p)

Alistair Howell, Sarah Dubrac, Kasper Krogh Andersen, David Noone, Juliette Fert, Tarek Msadek, Kevin Devine
Genes controlled by the essential YycG/YycF two-component system of Bacillus subtilis revealed through a novel hybrid regulator approach.
Mol Microbiol: 2003, 49(6);1639-55
[PubMed:12950927] [WorldCat.org] [DOI] (P p)

W Liu, S Eder, F M Hulett
Analysis of Bacillus subtilis tagAB and tagDEF expression during phosphate starvation identifies a repressor role for PhoP-P.
J Bacteriol: 1998, 180(3);753-8
[PubMed:9457886] [WorldCat.org] [DOI] (P p)

C Mauël, A Bauduret, C Chervet, S Beggah, D Karamata
In Bacillus subtilis 168, teichoic acid of the cross-wall may be different from that of the cylinder: a hypothesis based on transcription analysis of tag genes.
Microbiology (Reading): 1995, 141 ( Pt 10);2379-89
[PubMed:7581998] [WorldCat.org] [DOI] (P p)

C Mauël, M Young, A Monsutti-Grecescu, S A Marriott, D Karamata
Analysis of Bacillus subtilis tag gene expression using transcriptional fusions.
Microbiology (Reading): 1994, 140 ( Pt 9);2279-88
[PubMed:7952180] [WorldCat.org] [DOI] (P p)