ThrS

• Description: threonyl-tRNA synthetase (major)
  
  

Gene name	thrS
Synonyms
Essential	no
Product	threonyl-tRNA synthetase (major)
Function	translation
Gene expression levels in SubtiExpress: thrS
Metabolic function and regulation of this protein in SubtiPathways: tRNA charging
MW, pI	73 kDa, 5.214
Gene length, protein length	1929 bp, 643 aa
Immediate neighbours	ysaA, ytxC
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

translation

This gene is a member of the following regulons

T-box

The gene

Basic information

• Locus tag: BSU28950

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + L-threonyl-tRNA(Thr) (according to Swiss-Prot)

• Protein family: class-II aminoacyl-tRNA synthetase family (according to Swiss-Prot)

• Paralogous protein(s): ThrZ, one of the two proteins has to be present for viability PubMed

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:Cys573 is S-bacillithiolated by NaOCl stress PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 1TJE (from Escherichia coli, 44% identity, 63% similarity) PubMed

• UniProt: P18255

• KEGG entry: [3]

• E.C. number: 6.1.1.3

Additional information

• subject to Clp-dependent proteolysis upon glucose starvation PubMed

Expression and regulation

• Operon: thrS PubMed

• Expression browser: thrS PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced by threonine limitation (T-box) PubMed
  • expression transiently increases in the forespore PubMed

• Regulatory mechanism:
  • T-box: RNA switch, transcriptional antitermination PubMed

• Additional information: subject to Clp-dependent proteolysis upon glucose starvation PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: