NusB

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  • Description: probable transcription termination

Gene name nusB
Synonyms yqhZ
Essential no
Product unknown
Function unknown
Gene expression levels in SubtiExpress: nusB
MW, pI 14 kDa, 4.757
Gene length, protein length 393 bp, 131 aa
Immediate neighbours folD, yqhY
Sequences Protein DNA DNA_with_flanks
Genetic context
NusB context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
NusB expression.png
























Categories containing this gene/protein

transcription

This gene is a member of the following regulons

PurR regulon, stringent response

The gene

Basic information

  • Locus tag: BSU24320

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: nusB family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation:
    • repressed in the presence of purine nucleotides (PurR) PubMed
    • RelA dependent downregulation (Class I) during stringent response PubMed
  • Regulatory mechanism:
    • PurR: transcription repression (molecular inducer: PRPP) PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Geoff P Doherty, Donna H Meredith, Peter J Lewis
Subcellular partitioning of transcription factors in Bacillus subtilis.
J Bacteriol: 2006, 188(11);4101-10
[PubMed:16707701] [WorldCat.org] [DOI] (P p)

Christine Eymann, Georg Homuth, Christian Scharf, Michael Hecker
Bacillus subtilis functional genomics: global characterization of the stringent response by proteome and transcriptome analysis.
J Bacteriol: 2002, 184(9);2500-20
[PubMed:11948165] [WorldCat.org] [DOI] (P p)

H H Saxild, K Brunstedt, K I Nielsen, H Jarmer, P Nygaard
Definition of the Bacillus subtilis PurR operator using genetic and bioinformatic tools and expansion of the PurR regulon with glyA, guaC, pbuG, xpt-pbuX, yqhZ-folD, and pbuO.
J Bacteriol: 2001, 183(21);6175-83
[PubMed:11591660] [WorldCat.org] [DOI] (P p)

D J Ebbole, H Zalkin
Interaction of a putative repressor protein with an extended control region of the Bacillus subtilis pur operon.
J Biol Chem: 1989, 264(6);3553-61
[PubMed:2536750] [WorldCat.org] (P p)