FolD

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  • Description: methylenetetrahydrofolate dehydrogenase (NADP)

Gene name folD
Synonyms yqiA
Essential yes PubMed
Product methylenetetrahydrofolate dehydrogenase (NADP)
Function formylation of Met-tRNA(fMet)
Gene expression levels in SubtiExpress: folD
MW, pI 30 kDa, 5.6
Gene length, protein length 849 bp, 283 aa
Immediate neighbours yqiB, nusB
Sequences Protein DNA DNA_with_flanks
Genetic context
FolD context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
FolD expression.png
























Categories containing this gene/protein

translation, essential genes

This gene is a member of the following regulons

PurR regulon

The gene

Basic information

  • Locus tag: BSU24310

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: 5,10-methylenetetrahydrofolate + NADP+ = 5,10-methenyltetrahydrofolate + NADPH (according to Swiss-Prot)
  • Protein family: tetrahydrofolate dehydrogenase/cyclohydrolase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation:
    • repressed in the presence of purine nucleotides (PurR) PubMed
  • Regulatory mechanism:
    • PurR: transcription repression (molecular inducer: PRPP) PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Yann Duroc, Carmela Giglione, Thierry Meinnel
Mutations in three distinct loci cause resistance to peptide deformylase inhibitors in Bacillus subtilis.
Antimicrob Agents Chemother: 2009, 53(4);1673-8
[PubMed:19171795] [WorldCat.org] [DOI] (I p)

H H Saxild, K Brunstedt, K I Nielsen, H Jarmer, P Nygaard
Definition of the Bacillus subtilis PurR operator using genetic and bioinformatic tools and expansion of the PurR regulon with glyA, guaC, pbuG, xpt-pbuX, yqhZ-folD, and pbuO.
J Bacteriol: 2001, 183(21);6175-83
[PubMed:11591660] [WorldCat.org] [DOI] (P p)