GndA

• Description: NADP-dependent phosphogluconate dehydrogenase
  
  

Gene name	gndA
Synonyms	yqjI
Essential	no
Product	NADP-dependent phosphogluconate dehydrogenase
Function	pentose phosphate pathway
Gene expression levels in SubtiExpress: gndA
Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism
MW, pI	51 kDa, 5.076
Gene length, protein length	1407 bp, 469 aa
Immediate neighbours	zwf, polY1
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

carbon core metabolism, phosphoproteins

The gene

Basic information

• Locus tag: BSU23860

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: 6-phospho-D-gluconate + NADP⁺ = D-ribulose 5-phosphate + CO₂ + NADPH (according to Swiss-Prot)

• Protein family: 6-phosphogluconate dehydrogenase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information: Reversible reaction PubMed

• Domains:

• Modification: phosphorylation on (Thr-15 OR Thr-17) PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 2W8Z (from Geobacillus stearothermophilus, 83% identity, 92% similarity) PubMed

• UniProt: P80859

• KEGG entry: [3]

• E.C. number: 1.1.1.44

Additional information

It contains a cysteine on the active site. The enzyme is a dimer. PubMed

Expression and regulation

• Operon: polY1-gndA (according to DBTBS)

• Expression browser: gndA PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant: GP1514 (gndA::kan), available in Stülke lab

• Expression vector:
  • pGP1777 (for expression, purification in E. coli with N-terminal Strep-tag, in pGP172, available in Stülke lab)
  • pGP1789 (N-terminal Strep-tag, for SPINE, purification from B. subtilis, in pGP1389) (available in Stülke lab)
  • GP1408 (gndA-Strep (spc)) & GP1410 (gndA-Strep (cat)), purification from B. subtilis, for SPINE, available in Stülke lab

• lacZ fusion:

• GFP fusion:

• two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab

• Antibody:

• FLAG-tag construct:
  • GP1403 (spc, based on pGP1331), available in the Stülke lab
  • GP1408 (kan, resistance cassette exchange in GP1403), available in the Stülke lab

References

Additional publications: PubMed

Scott Cameron, Viviane P Martini, Jorge Iulek, William N Hunter
Geobacillus stearothermophilus 6-phosphogluconate dehydrogenase complexed with 6-phosphogluconate.
Acta Crystallogr Sect F Struct Biol Cryst Commun: 2009, 65(Pt 5);450-4
[PubMed:19407374] [WorldCat.org] [DOI] (I p)

Boris Macek, Ivan Mijakovic, Jesper V Olsen, Florian Gnad, Chanchal Kumar, Peter R Jensen, Matthias Mann
The serine/threonine/tyrosine phosphoproteome of the model bacterium Bacillus subtilis.
Mol Cell Proteomics: 2007, 6(4);697-707
[PubMed:17218307] [WorldCat.org] [DOI] (P p)