GutB

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  • Description: D-sorbitol dehydrogenase

Gene name gutB
Synonyms
Essential no
Product D-sorbitol dehydrogenase
Function glucitol utilization
Gene expression levels in SubtiExpress: gutB
Metabolic function and regulation of this protein in SubtiPathways:
Sugar catabolism
MW, pI 38 kDa, 5.213
Gene length, protein length 1059 bp, 353 aa
Immediate neighbours gutR, gutP
Sequences Protein DNA DNA_with_flanks
Genetic context
GutB context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
GutB expression.png




























Categories containing this gene/protein

utilization of specific carbon sources

This gene is a member of the following regulons

GutR regulon

The gene

Basic information

  • Locus tag: BSU06150

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: L-iditol + NAD+ = L-sorbose + NADH (according to Swiss-Prot)
  • Protein family: zinc-containing alcohol dehydrogenase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulatory mechanism: GutR: transcriptional activation PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Shouji Watanabe, Miyuki Hamano, Hiroshi Kakeshita, Keigo Bunai, Shigeo Tojo, Hirotake Yamaguchi, Yasutaro Fujita, Sui-Lam Wong, Kunio Yamane
Mannitol-1-phosphate dehydrogenase (MtlD) is required for mannitol and glucitol assimilation in Bacillus subtilis: possible cooperation of mtl and gut operons.
J Bacteriol: 2003, 185(16);4816-24
[PubMed:12897001] [WorldCat.org] [DOI] (P p)

R Ye, S L Wong
Transcriptional regulation of the Bacillus subtilis glucitol dehydrogenase gene.
J Bacteriol: 1994, 176(11);3314-20
[PubMed:8195086] [WorldCat.org] [DOI] (P p)

K Ng, R Ye, X C Wu, S L Wong
Sorbitol dehydrogenase from Bacillus subtilis. Purification, characterization, and gene cloning.
J Biol Chem: 1992, 267(35);24989-94
[PubMed:1460002] [WorldCat.org] (P p)